Nucleic acid amplification testing

Tests that allow rapid identification of chlamydial antigens in genital secretions are the direct fluorescent antibody test, the enzyme immunoassay (requires just 30 minutes for results), the DNA hybridization probe and nucleic acid amplification tests. 

Nucleic acid amplification testing nucleic acid testing Nucleotide-binding domain... 

Centers for Disease Control and Prevention. Nucleic acid amplification tests for tuberculosis. MMWR 1996 45 950-2. 

Nucleic acid probe methods which rely on the specificity of nucleic acid hybridization reaction, combined with the powerful methods of nucleic acid amplification, are expected to spawn many new diagnostic tests which may be useful in the detection and treatment of infectious diseases, cancer, and inherited disorders. The impact of development and applications of diagnostic tests based on nucleic acid probes and on Mab technology wiU be felt increasingly as deeper understanding of diseases at a molecular level is gained. 

Battle TJ, Golden MR. Evaluation of laboratory testing methods for Chlamydia trachomatis infection in the era of nucleic acid amplification. J Clin Microbiol 2001 39 2924-2927. 

All of these tasks can now be addressed with a family of novel tests collectively named product-enhanced reverse transcriptase (PERT) assays. PERT assays combine the broad detection range of RT tests with the high sensitivity of nucleic acid amplification procedures. PERT assays are based on the selective enhancement, by polymerase chain reaction (PCR) or one of the various... 

Nucleic acid-based testing for infectious diseases, as for other clinical conditions, usually involves three major steps specimen processing, nucleic acid amplification, and... 

Viral replication is detected by two types of tests target nucleic acid amplification based tests and hybridization (signal amplification) based tests. 

We have exemplified BART applications in molecular IVDs and demonstrated its compatibility with various amplification methods, its ability to detect and quantify different targets, to cope with crude sample preparations and to provide rapid results under challenging conditions. Overall, BART is a universal reporter system for any molecular in vitro diagnostic tests based on isothermal nucleic acid amplification techniques. 

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