Nuclear transfer

McMahon Presumably the nuclear transfer experiments with somatic nuclei should give you some insight. 

A number of alternative approaches are being developed that may overcome some of these issues. Replication-defective retroviral vectors are available that will more consistently (a) deliver a chosen gene into cells and (b) ensure chromosomal integration of the gene. A second innovation is the application of nuclear transfer technology. 

Blasting charges, 70 734 Blastomeres, nuclear transfer from,... 

Nuclear steam cycles, 23 234-236 Nuclear steam generators, 23 217 Nuclear transfer, embryo cloning by, 12 451—452... 

Somatic cell nuclear transfer, species and cell types used for, 12 452t Somatic cells... 

Hlfoo Oocytes In the zygote up to midblastula transition Replaces somatic HI during somatic cell nuclear transfer RI ... 

Colin M, Moritz S, Fontanges P, et al. The nuclear pore complex is involved in nuclear transfer of plasmid DNA condensed with an oligolysine-RGD peptide containing nuclear localisation properties. J Biol Chem 2001 8(21) 1643-1653. 

Stice, S.L., Strelchenko, N.S., Keefer, C.L., and Mathews, L. (1996). Plmipotent bovine embryonic cell lines direct embryonic development following (nuclear transfer. Biol. Reprod. 54,100-110. 

Korean scientists improve success rate of human adult nuclear transfer to embryonic cells by 10 fold... 

Somatic nuclear transfer (SNT)—Mechanical transfer of the nucleus of a body cell to replace the nucleus of an animal or human egg. 

BOX 15.1. BASIC CONCEPT OF NUCLEAR TRANSFER TECHNOLOGY IN ANIMAL CLONING... 

Figure 15.1. Schematic representation of nuclear transfer strategy to produce genetically identical offspring. (Adapted from PLL-Therapeutics)...

Campbell, K.H., J. McWhir, W.A. Ritchie, and I. Wihnut, Sheep cloned by nuclear transfer from a cultured cell line. Nature, 1996. 380(6569) 64-6. 

Wakayama, T., V. Tabar, I. Rodriguez, A.C. Perry, L. Studer, and P. Mombaerts, Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer. Science, 2001. 292(5517) 740-3. 

Over the past decade, significant progress has been made in stem cell research the derivation of ESCs from human blastocysts [9], the development of somatic cell nuclear transfer technology [10] and the confirmation that neurogenesis occurs in the adult... 

Wakayama, T. (2006). Establishment of nuclear transfer embryonic stem cell lines from adult somatic cells by nuclear transfer and its application. Ernst Sobering Res Found Workshop, 60, 111-23. 

Meissner, A., Jaenisch, R. (2006). Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx2-deficient blastocysts. Nature, 439, 212-5. 

Hurlbut, W.B. (2005). Altered nuclear transfer as a morally acceptable means for the procurement of human embryonic stem cells. Perspect Biol Med, 48, 211-28. 

Melton, D.A., Daley, G.Q., Jennings, C.G. (2004). Altered nuclear transfer in stem-cell research - a flawed proposal. N Engl J Med, 351, 2791-2. 

Wakayama, S., Jakt, M.L., Suzuki, M., Araki, R., Hikichi, T., Kishigami, S., Ohta, H., Van Thuan, N., Mizutani, E., Sakaide, Y., Senda, S., Tanaka, S., Okada, M., Miyake, M., Abe, M., Nishikawa, S., Shiota, K., Wakayama, T. (2006). Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts. Stem Cells, 24,2023-33. 

Researchers at Scotland s Roslin Institute reported that they had cloned a sheep-named Dolly-from the cell of an adult ewe. Polly, the first sheep cloned by nuclear transfer technology bearing a human gene, appeared later... 

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