Nuclear speckles

Sassone-Corsi You mentioned that phyB interacts with cry2 in the nuclear speckles. Is there colocalization ... 

Gigena MS, Ito A, Nojima H et al (2005) A B56 regulatory subimit of protein phosphatase 2A localizes to nuclear speckles in cardio-myocytes. Am J Physiol Heart Circ Physiol 289 H285-H294... 

After identification ofthe spliceosome as the pladienohde target, sphcing inhibition under pladienohde B treatment could be confirmed by polymerase chain reaction (PCR)-based assays against sphced and unspliced isoforms of several genes. Furthermore, it was observed that pladienohde application led to rearrangement and enlargement of nuclear speckles. 

Spector, D.L. and Lamond, A.I. (2011) Nuclear speckles. Cold Spring Harbor Perspect Biol., 3 (2). 

Boronenkov, IV, Loijens, JC, Umeda, M and Anderson, RA (1998) Phosphoinositide signaling pathways in nuclei are associated with nuclear speckles containing pre-mRNA processing factors. Mol Biol Cell, 9, 3547-3560. 

Recently, a photoactivatable variant from Aequoria victoria green fluorescent protein (pa-GFP) was reported (Patterson and Lippincott-Schwartz 2002), yielding an increase in fluorescence emission intensity (at k 520 nm) by a factor of 100 when excited at k 488 nm after spectral activation at A. 408 nm. This phenomenon is due to an internal photoconversion process in the protein and allows spectral photoactivation of this protein in a very local way such as in the nucleus of a living cell (Post et al. 2005). In tobacco BY-2 protoplasts, we transiently co-expressed pa-GFP or pa-GFP fusion proteins and red-fluorescent protein (DsRed)-tagged prenylated Rab acceptor 1 (Pral At2g38360), a membrane protein that localizes in speckles around the nuclear envelope. The DsRed transfection allows proper cell identification and visualization before activation (via Pral -DsRed fluorescence). After pa-GFP... 

Evidently, ANA (first described by G.J. Friou et al. in 1957) belong to the natural autoantibodies which are continuously stimulated by a still unknown mechanism (possibly a persisting agent) in certain diseases. However, this antibody phenomenon is inconsistent and neither organ- nor disease-specific. ANA can be visualized by immunofluorescence in cryostat tissue sections of the rat liver and rat kidney as a homogeneous (s. fig. 5.10), speckled (s. fig. 5.11), circular or nuclear fluorescence pattern. This allows conclusions to be drawn as to the specificity of antinuclear antibodies and in some cases as to the nature of the underlying disease as well. 

Speckled Acidic nuclear protein Rheumatoid arthritis... 

Almost all patients have high titres of positive fluorescent antinuclear antibodies with a speckled pattern and very high titres of anti-RNP, a component of extractable nuclear antigen. 

---