Pre-mRNA processing

Huang, Y., Winder, K.M. and Carmichael, G.G. (1999) Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing. EMBO J., 18,1642-1652. 

Figure 29.34 The CTD Coupling transcription to pre-mRNA processing. The transcription factor TFIIH phosphorylates the car boxy I-terminal domain (CTD) of RNA polymerase II, signaling the transition from transcription initiation to elongation. The phosphorylated CfD binds factors required for pre-mRNA capping, splicing, and polyadenyiation. These proteins are brought in close proximity lo their sites of action on the nascent pre-mRNA as it is transcribed during elongation.

One feature of genes that is very important to our interests in pre-mRNA processing is the degree of dispersal of the RNA transcripts. The two genes in... 

A further study presented evidence that splicing does influence transcription. In this case, SSA derailed proper pre-mRNA processing, by abolishing RNAPII stopping at the 3 end of the message, which in turn enhanced pre-mRNA leakage into the cytoplasm [47]. 

Moore, M.J. and Proudfoot, N.J. (2009) Pre-mRNA processing reaches back to transcription and ahead to translation. Cell, 136 (4), 688-700. 

Pre-mRNA processing is performed by a complex called the spliceo-some. This is composed of small molecules of RNA known as small nuclear RNA (snRNA) and proteins to form several different types of small nuclear rihonucleoprotein (snRNP usually pronounced snurps ). One type of snRNP binds to the 5 end (or left flank) of the intron while another type of snRNP binds to the 3 end (or right flank) of the intron. 

Patients with SLE produce autoantibodies against snRNPs and suffer the effects of aberrant pre-mRNA processing. 

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