Nuclear particles purification

Fractionation of proteins from rat-liver nuclear 30S-particles Purification of trypsin studies of the affinity characteristics of the adsorbent... 

Inomata, Y., Kawaguchi, H., Hiramoto, M., Wada, T., Handa, H. (1992). Direct purification of multiple ATF/E4TF3 polypeptides from HeLa cell crude nuclear extracts using DNA-carrying affinity latex particles. Anal. Biochem., 206, 109-114. 

The in vitro assay for U snRNP import utilizes fluorescently labeled affinity-purified UsnRNP particles. HeLa nuclei can be prepared using the method of Dignam et al. (1983). Nuclear extracts are made and the U snRNPs are purified from the nuclear extract in a one-step purification procedure using an antibody... 

Bach, M., Bringmann, P., and Ltihrmann, R. (1990). Purification of small nuclear ribonucleoprotein particles with antibodies against modified nucleosides of small nuclear RNAs In Methods in Enzymology (J. E. Dahiberg and J. N. Abelson, eds.), Vol. 181, pp. 232-257. Academic Press, San Diego, CA. 

The purification of D-RNA-containing particles. In our first work on the nuclear D-RNP, ultracentrifugation in a sucrose gradient was used for the purification of the particles from the other components of the extract (Samarino at al., 1965a). After... 

Thus, for the purification of nuclear D-RNA from extracts, density gradient ultracentrifugation without prior pelleting is the best procedure at the moment. The material obtained from the peak with a sedimentation coefficient equal to 308 represents D-RNP particles of 90 to 95 percent purity and may be used for the study of the properties or function of the particles. 

In a nuclear power station, there are several ion-exchange systems for water clean-up. In a typical BWR there are purification circuits after the steam condenser, in a small stream from the reactor vessel and for the water in the fuel storage pool. In a pressurized water reactor (PWR), part of the coolant in the primary circuit is withdrawn, cooled, and pumped through an ion-exchange filter. As indicated by the word filter, these ion exchangers are also used to remove particles from the liquids. They often consist of mixed cation and anion exchangers that are very finely ground bead sizes of 400 mesh are common. 

Swanson, M. S., and Dreyfuss, G. (1988) Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol. Cell. Biol. 8, 2237-2241. 

Uranium-233 (V, = -1.59 10 years) is formed through neutron capture by Th (mainly in thorium-fueled nuclear reactors) followed by emission of two beta particles. Alternatively, may be formed by alpha decay of Np that in turn is produced through a sequence of nuclear reactions in uranium-fueled reactors. The °Th decay product of is the relatively short-lived (t = -7880 years) so the ratio between these nuclides is linear for thousands of years after purifications. Alpha spectrometry and mass spectrometry can be used for measuring the Th/ U ratio. In special cases, this pair of nuclides cau serve as a chrouometer for nuclear proliferation, particularly if fissile is produced in reactors fueled with thorium. 

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