Non-Specific Degradation

Another possible explanation for the unusual ratios is that polypeptide F, which is used to standardize the molar ratios, is unstable in the sense that it is non-specifically degraded diaring the course of infection. A low value for F would tend to increase the A F ratio. Thus far, no-one has reported that polypeptide F is unstable and no change in its molar concentration was detected even when the viral proteins were isolated in the... 

Specific effluents have also been subjected to WRF-mediated remediation studies. Decolourization, dechlorination and detoxification of highly toxic bleach plant effluents derived from the pulp and paper industry have been reported [26-28], while degradation and decolourization of synthetic dyes due to the non-specificity of the LMEs have been widely documented [29, 30], Likewise, treatment of the acidic, phenolic-rich olive oil mill wastewater has shown COD reduction, decolourization and dephenolization [31-34],... 

Albumin has a molecular mass of approximately 66 000 and is synthesized at a rate of about 12 g, equal to 3% of total body albumin, per day to replace that which is degraded or lost. Impaired albumin synthesis and therefore a low plasma albumin concentration, is a hallmark of chronic liver disease. Several functions can be ascribed to albumin including osmotic (oncotic) pressure regulation of the plasma and a non-specific transport protein for ligands such as calcium, fatty acids, drugs and bilirubin. 

Recent developments have led to agents with a built-in functional group that allows more rapid metabolism. Initially, the presence of ester groupings, as in suxamethonium, allowed fairly rapid metabolism in the body via esterase enzymes that hydrolyse these linkages. The enzyme involved appears to be a non-specific serum acetylcholinesterase (see Box 13.4). Even better is the inclusion of functionalities that allow additional degradation via an elimination reaction. Such an agent is atracurium. 

Equilibrium Dialysis Standard ED High-throughput, 96-well format. Long incubation time - compound instability and plasma degradation. Issues of pH drift and osmotic volume shifts (can be corrected for). Membrane adsorption/non-specific binding. [30-32]... 

The non-specific alkaline phosphatases present in bone and calcifying cartilage have several properties in common. The ATPases concerned in the formation of different hard tissues seem to be isozymes. It could be shown that two enzymes capable of degrading ATP exist. One of them can be inhibited by levamisole and R 8231 and is probably identical with non-specific alkaline phosphates. The activity of the other enzyme, tentatively named Ca-ATPase , is dependent on the presence of Ca2+ or Mg2+ and is activated by these ions. The Ca-ATPase is unaffected by ouabain and ruthenium red. It may be speculated that the Ca-ATPase is concerned with the transmembranous transport of Ca2+-ions to the mineralization front229. ... 

Cationic lipids and polymers (including dendrimers) deliver DNA and siRNA into cells via cargo compaction to give lipoplexes or polyplexes (or dendriplexes), respectively, of various sizes which are then taken up via non-specific endocytosis (see Fig. 1). Once these complexes have been endocytosed the cargo has to be released into the cytoplasm to avoid degradation by the lysosomes. This escape is based upon the ability of the carrier to dismpt endosomal integrity [29-33] and is a critical step in the process. Once in the cytoplasm, the genetic material needs to be... 

---