Neomycin sulphate

Hirtz et al demonstrated the hygroscopic nature of neomycin sulphate using a thermogravimetric technique. In a more recent study Russian workers18 determined the adsorption of water by neomycin sulphate at temperatures of 23, 90 100 C. Table 3 gives the results... 

Using a method based on the filtration of air through a compressed layer of powder Ezerskii et al determined the visible specifie.surface area of neomycin sulphate to be 2.6 m2g. ... 

Solvent Neomycin B hydrochloride Neomycin sulphate Neomycin undecylenate Neomycin oleate Neomycin stearate... 

Kigel et al have reported the density of 6 18% aqueous neomycin sulphate solutions over the temperature range 20° to 80°C. 

Surface Tension Values for Neomycin Sulphate Solutions... 

Japanese workers 5 have described the stability of bulk fradiomycin (neomycin B)sulphate which was shown to have retained 99% of its microbiological potency after storage for 24 months. Neomycin sulphate powder has been reported to be stable for at least 3 years at 20°C 298,305 ... 

Neomycin sulphate may also be heated at 110°C for 10 hours, during dry-heat sterilisation, without loss of potency, though some degree of yellowing is apparent298. 

Reconstituted aqueous suspensions of neomycin sulphate, containing tragacanth and sugar have been shown to be stable for 10 days when stored at 4°C but some loss of potency was observed when the suspension was exposed to daylight- . Non-aqueous suspensions containing peanut oil and lanolin have been reported298 which are stable for 1 year at 20°C. 

An alternative method for determining the sulphate content of neomycin sulphate has been reported by Roets and Vanderhaeghel22. 

The use of differential pulse polarography to determine neomycin sulphate in aqueous solutions has been described by Siegerman et all2. Following acid hydrolysis of neomycin, the solution was adjusted to pH 4 and 0.1M acetate... 

Recently an isotope dilution method has been reported O for assaying neomycin sulphate. However, it is first necessary to prepare 14C-labelled neomycin sulphate. This is accomplished by adding l4C-labelled glucose to a small-scale fermentation of 5. nad-iat. l4C-labelled neomycin can then be extracted by solvent-extraction or by ion-exchange chromatography. 

The neomycin molecule does not exhibit fluorescence but following suitable de-rivatisation two spectrofluorimetric determinations have been described. Maeda et al l reported the complexation of simple hexosamines with pyridoxal and zinc ions to result in a fluorescent derivative. Applying this procedure to neomycin, Simpsonl32 demonstrated a linear response over the concentration range 0-50 yg/ml of neomycin sulphate. 

Thin-layer chromatography has been used by Foppiano and Brown229 to assay the total neomycin B and C content of neomycin sulphate. The neomycin zone was scraped off the plate and reacted with orcinol/ferric chloride reagent, the absorbance of the resulting colour being measured at 665nm. By this procedure a precision of - 2% was achieved. 

A comparison of the G.L.C. and microbiological (agar-diffusion) assays has been reported by Tsuji et al239 who showed the neomycin content of neomycin sulphate powders determined by the G.L.C. method, to correlate well with values obtained by the microbiological assay when it is assumed neomycin C has 35% of the biological activity of neomycin B. 

Dose. As an intestinal antiseptic, up to 12 g of neomycin sulphate daily, by mouth it is also applied topically. 

PROP White odorless powder. Bp Degrades before boiling. Sol in water SYNS BIOSOL VETERINARY FRADIOMYCIN SULFATE LIDAMYCIN CREME MYCAIFRADIN SULFATE MYCIFRADIN-N MYCIGIENT NEOBIOTIC NEO-MANTLE CREME NEOMIX NEOMYCINE SULFATE NEOMYCIN SULPHATE OTOBIOTIC QUINTESS-N USAF CB-19... 

Nitrate reductase, 1.9.6.1 Mo-pterin, Fe—S Nitrate PGE, neomycin sulphate [252]... 

Kodym A, Marcinkowski A, Kukula H. Technology of eye drops containing aloe (Aloe arborescens M-Liliaceae) and eye drops containing both aloe and neomycin sulphate. Acta Pol Pharm 2003 60(1) 31-39. 

Neo-Mercazole carbimazole. neomycin [ban, inn] (neomycin suiphate Mycifradin ) is an (aminoglycoside) ANTIBIOTIC composed of a complex of neomycin A, B and C - early members of the aminoglycoside family produced by the mould Streptomyces fradiae. It has broad-spectrum antibacterial properties but is too toxic to be injected, though it can be used in treating some superficial bacterial infections, and is occasionally taken by mouth to reduce the levels of bacteria in the gut (it is not absorbed), neomycin B framycetin sulphate, neomycin sulphate neomycin, neopentanetetrayl nicotinate niceritrol. Neoral cyclosporine. 

In the treatment of secondarily infected dermatoses, which are usually colonized with streptococci, staphylococci, or both, compound antiinflammatory and antibacterial preparations are indicated when the underlying disorder has been diagnosed. Corticosteroids do not appear to inhibit the activity of antibacterial agents in compound preparations for topical application. Examples of topical compound preparations include betamethasone valerate-fusidic acid gel (for dogs), betamethasone sodium phosphate-neomycin sulphate cream (for dogs and cats), prednisolone-neomycin sulphate-nitrofurazone ointment (for horses, dogs and cats). The use of compound preparations is contra-indicated in pregnant animals. 

B. Neomycin INN, Neomycin Sulphate BAN, Neomycin Sulphate USAN,... 

Neomycin sulphate Silica gel. 3% NH4OH-acetone (80 -f 20) Reference chromato- gram Colour reagent served as eluent photometry after elution... 

To see if this loss was due to instability of active renin at 37°C or to a possible inhibitory effect of plasma on renin (24), partially purified active kidney renin was incubated at 37°C in the pH 7.4 phosphate-saline buffer (4) that contained neomycin sulphate. 

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