Neocuproine

A -Benzoyl-i V-phenyl hydroxylamine 440 Nebuliser-burner system 785 Neocuproin 178... 

Figure 5. pH activity profile of PGE on polygalacturonate. Mcllvaine buffers were used in the pH range from 2.5 to 5,5, Reducing sugars were detected by the neocuproine method. PGE (2 mg/ml) was 80-times diluted and incubated with 500 pi of a 0.25% (w/v) polygalacturonate solution, at SO C. 

Peris-Tortajada, M., Puchades, R., and Maquieira, A. (1992). Determination of reducing sugars by the neocuproine method using flow injection analysis. Food Chem. 43,65-69. 

Apak R, Guylu K, Mustafa 6 and Karademir SE. 2004. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine CUPRAC Method. J Agric Food Chem 52(26) 7970-7981. 

A lipophilic copper complex with neocuproine has been found to increase the toxicity of a trichlorophenol in bacteria, probably due to increased transport of Cu over the membrane [229],... 

Zhu, B.-Z. and Chevion, M. (2000). Copper-mediated toxicity of 2,4,5-trichlor-ophenol biphasic effect of the copper(I)-specific chelator neocuproine, Arch. Biochem. Biophys., 380, 267-273. 

The neocuproine method for the measurement of the cuprous oxide is more sensitive than the phosphomolybdic acid reagent and uses 2,9-dimethyl-1, 10-phenanthroline hydrochloride (neocuproine), which produces a stable colour and is specific for cuprous ions. 

NdjlCufpballj 23H2O, 43 208-210 Negative ion spectra, 28 7 Neocuproine copper complexes, 45 289 Neodymium carbides, 11 200 dibromide, 20 4 dichloride, 20 4 preparation of, 20 8 properties of, 20 15-18 diiodide, 20 4... 

Various UV/Vis spectroscopic studies have been described by Reedijk and co-workers, aimed at identifying the proposed dicopper species with bridging methoxide and phenoxide ligands (compare 8) in the absence of neocuproine. Notion is made of two bands, appearing at 420 and 450cm which were attributed to the formation of /z-OMe and /z-phenoxo bridges, respectively. 

Apak R, Giiglii K, Ozytirek M, Karademir SE. Novel Total Antioxidant Capacity Index for Dietary Polyphenols and Vitamins C and E, Using Their Cupric Ion Reducing Capability in the Presence of Neocuproine CUPRAC Method. Journal of Agricultural and Food Chemistry. 2004 52 (26) 7970-7981. 

Iodothiophene was coupled with thiophenol in the presence of a copper-neocuproin catalyst to give 2-phenylthio-thiophene in excellent yield. (6.84.)114... 

Several tetrahedral cuprous phenanthroline complexes, known inhibitors of transcription, were tested against integrase and shown to be reasonably effective inhibitors [56] IC50 values in the range of 1-10 jiMwere determined (for example, the neocuproine-Cu+ complex, XII). Analyses of the mode of inhibition demonstrated that these compounds act noncompetitively, and that inhibition does not correlate with inhibition of DNA binding. Thus, it has been proposed that these metal chelates may act at a site distant from the active site, or perhaps in the context of an enzyme-DNA complex. 

H. Diehl and G. F. Smith, The Copper Reagents-Cuproine, Neocuproine and Bathocuproine. Smith Chem. Co., Columbus, Ohio, 1958. 

In the preceding iron determination, the results would be about 10% high because serum copper also forms a colored complex with ferrozine. Interference is eliminated if neocuproine or thiourea is added. These reagents mask Cu+ by forming strong complexes that prevent Cu+ from reacting with ferrozine. 

Neocuproine Tightly bound complex with little... 

D. Cu+ reacts with neocuproine to form the colored complex (neocuproine)2Cu+, with an absorption maximum at 454 nm. Neocuproine is particularly useful because it reacts with few other metals. The copper complex is soluble in 3-methyl-1-butanol (isoamyl alcohol), an organic solvent that does not dissolve appreciably in water. In other words, when isoamyl alcohol is added to water, a two-layered mixture results, with the denser water layer at the bottom. If (neocuproine)2Cu+ is present, virtually all of it goes into the organic phase. For the purpose of this problem, assume that the isoamyl alcohol does not dissolve in the water at all and that all the colored complex will be in the organic phase. Suppose that the following procedure is carried out ... 

Next, 10.00 mL of the solution are transferred to flask B and treated with 10.00 mL of a reducing agent to reduce all Cu2+ to Cu+. Then 10.00 mL of buffer is added to bring the pH to a value suitable for complex formation with neocuproine. 

After that, 15.00 mL of this solution are withdrawn and placed in flask C. To the flask are added 10.00 mL of an aqueous solution containing neocuproine and 20.00 mL of isoamyl alcohol. After the mixture has been shaken well and the phases allowed to separate, all (neocuproine)2Cu+ is in the organic phase. 

What is the purpose of neocuproine in the serum iron analysis ... 

Dunkley 1959A) and neocuproine (Smith and Dunkley 1962B), among others (Samuelsson 1967), have also proven their effectiveness as inhibitors of autoxidation. 

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