N-Hydroxy succinimide

PGIP, purified fi om P.vulgaris hypocotyls [11], was immobilized to the sensor ch via amine coupling. A continuous flow of HBS buffer (5 pl/min) was mantained over the sensor surface. The carboxylated dextran matrix of the sensor surface was first activated by a 6-min injection of a mixture of N-hydroxy-succinimide and N-ethyl-N - (3-diethylaminopropyl) carbodiimide, followed by a 7-min injection of PGIP (lOng/pl in 10 mM acetate, pH 5.0). Hie immobilization procedure was con leted by a 7-min injection of 1 M ethanolamine hydrochloride to block the remaining ester groups. 

Label amino function in DNA probe with N-hydroxy succinimide derivatives of acridinium esters. 

Radford GM, Ariatti M, Hawtrey AO. Cholesterylsuccinyl-N-hydroxy-succinimide as a cross-linking agent for the attachment of protein to liposomes. Biochem Pharmacol 1991 41 307. 

Fig. 8 Examples of homobifimctional crosslinkers a bisimidate and b N-hydroxy-succinimide ester...

Di- and oligo-peptides with a terminalo-aminophosphonous acid residue have been prepared by coupling with N-hydroxy succinimide esters of N-benzyloxycarbonylamino acids or peptides. 

Undecylenic acid has also been shown to react with the surface preferentially at the alkene end, leaving the terminal carboxylic acid group free for further reaction [53], This result was somewhat unexpected as the Si-H sites are considered to be somewhat acidic and the oxophilic nature of silicon should thermodynamically favor reaction with the hydroxyl group of the acid. The preferential reactivity with the alkenyl end is consistent with a free radical, rather than a nucleophilic mechanism. The acid function can be activated with N-hydroxy succinimide (NHS) to facilitate coupling with amine tagged molecules. Schematically,... 

The surface matrix of a carboxymethylated sensor chip CMS (Pharmacia Biosensor, Uppsala, Sweden) was activated by injection of 35 pL of 0.05 M N-hydroxy-succinimide (NHS)/0.2 M N-ethyl-N (dimethylaminopropyl)carbodiimide (EDC). The BIAcore IFC forms four parallel flow cells on the sensor chip. Three of them were derivatized with 40 mM cystamin-dihydrochloride/ethanolamine in the ratios... 

Carbodiimides are widely used to mediate the attachment of biomarkers to polypeptides. Examples include carbodiimides with ferrocenyl substituents. Also, peptides are covalently modified with ferrocenecarboxylic acid using EDCCl and N-hydroxy-succinimide to promote the coupling to surface lysines. They also mediate the attachment of substituents to single walled nanotubes (SWNTs) and multiwalled nanotubes (MWNTs). Also, microdots are attached to virus molecules using a water soluble carbodiimide. The attachment of viral DNA to gold particles is used in the manufacture of a new type of vaccine. 

Figure 5-5. Radioactive labelling of proteins by treatment with the Bolton-Hunter reagent. The primary amino group of proteins can be modified by treatment with N-hydroxy-succinimide esters. The diagram illustrates the introduction of 125l by use of the Bolton-Hunter reagent, N-succinimidyl-3-(4-hydroxy-5-[125l]iodophenyl)-propionate.

Free Amine N-hydroxy succinimide ester (NHS) Amide (151, 152)... 

In order to functionalize the metal-coated substrates with MPA, they were immersed in an ethanolic solution of 0.02 M MPA. The MPA layer was activated using a solution of 0.002 M ethyl dimethylaminopropyl carbodiimide (EDC) and 0.005 M n-hydroxy succinimide (NHS) in 2-(Ai-morpholino) ethanesulfonic acid (MES) buffer solution (20 mM MBS, 0.1 M NaCl, pH5). The carboxylate groups of the MPA react with NHS in the presence of EDC to form NHS-esters which can then react with amine groups of proteins. After 15 min of activation, the metalized... 

Figure 2. Structures of the N-hydroxy succinimide (NHS) esters of selected nitroaromatic haptens, the hapten-bovine serum albumin (BSA) conjugates and the hapten-ovalbumin (OVA) conjugates.

In this procedure, a set of four isobaric amine-specific labeling reagents (114, 115, 116, or 117), consisting of a reporter group, a balance group, and a peptide-reactive group (e.g., N-hydroxy succinimide) are used to label... 

Active Esters. The synthesis of reactive esters of acylamino acids and their use in lengthening a peptide chain by reaction at the amino end is a justifiably popular method racemization is avoided, yields are good, and purification of the products is relatively easy. Esters of N-hydroxy-succinimide (l), N-hydroxypiperidine (ll) and 8-hydroxyquinoline (III) have received particular attention during I966. 

Treatment of (E)-5-(3-aminoprop-l-enyl)-2 -dUTP with the N-hydroxy-succinimide ester of 3-methylthiopropionic acid affords (224), which can be... 

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