Myeloma cell lines growth

Billadeau, D., Liu, P., Jelinek, D., Shah, N., LeBien, T. W. and van Ness, B. (1997). Activating mutations in the N- and K-ras oncogenes differentially affect the growth properties of the IL-6-dependent myeloma cell line ANBL6. Cancer Res. 57, 2268-2275. 

Zhang, X.G., Gu, J.J., Lu, Z.Y., Yasukawa, K., Yancopoulos, G.D., Turner, K., Shoyab, M., Taga, T., Kishimoto, T., Bataille, R. and Klein, B. (1994) Ciliary neurotrophic factor, interleukin 11, leukemia inhibitory factor, and oncosta-tin M are growth factors for human myeloma cell lines using the interleukin 6 signal transducer gpl30. J. Exp. Med. 179 1337-1342. 

Several s mtheses of nucleotidephospholipids have been reported recently. For example, phospholipid-araC conjugates have been prepared and tested as prodrugs of arotC as inhibitors of the growth of a murine myeloma cell line. The most effective inhibitor was [13, = 1, R = Me(CH2)i4] solubility difficulties may have affected the testing of other analogues. 

Passive antibody vaccines have been prepared up to now from human blood serum. Consequently, there has been no need for cultivation methods beyond vaccination and conventional harvest of antibody-containing blood from donors. Due to safety concerns over using human blood, passive vaccines will likely be monoclonal antibodies or cocktails thereof prepared in vitro by the cultivation of hybridoma or myeloma cell lines. This approach is under investigation for anti-HIV-1 antibodies [Emini et al., 1992]. Cultivation of these cell lines involves the same principles of animal cell cultivation as described above, with the exception that hybridomas can be less fastidious in nutritional requirements, and they do not require surface attachment for growth. These features will allow for defined serum-free media and simpler cultivation vessels and procedures. 

Another important aspect involved in the selection of transfected lines is the capacity to grow without physical support, since the scale-up of such processes is much simpler than those designed for growth of anchorage-dependent cells. Thus, cells that grow naturally in suspension are preferred, such as myeloma cells (Sp2/0 and NSO), or others that can be easily adapted to this form of cultivation, such as CHO and BHK (Chu and Robinson, 2001). 

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