Mutants of Chlamydomonas reinhardtii

Polle, J.E.W. et ah. Photosynthetic apparatus organization and function in wild type and a Chi b-less mutant of Chlamydomonas reinhardtii. Dependence on carbon source, Planta, 211, 335, 2000. 

Govindjee, M.J. Seufferheld, Nonphotochemical quenching of chlorophyll a Fluorescence Early history and characterization of two xanthophyll-cycle mutants of Chlamydomonas Reinhardtii, Funct. Plant Biol. 29, 1141-1155 (2002)... 

Antenna size reduction unfortunately is not trivial and current antenna size mutants of Chlamydomonas reinhardtii, for example, were not able to outperform the wild type (De Mooij et al, 2014). It is important to stress that only light absorption should be reduced and that the capacity to process photons and perform photosynthesis should remain unaltered. In other words, the number of photosystems must remain maximal and only the size of the antenna complex of the photosystems should be reduced (Formighieri et al, 2012 Wobbe and Remade, 2014). 

In the present study we have used the chi b-less mutant of Chlamydomonas reinhardtii strain cbnl-48 to clarify the role of chi b and carotenoids in the assembly of LHC II. The thylakoids of the chi b-less mutant present a distinct pigment composition and a specific LHC II polypeptide pattern. LHC II from thylakoid membranes of the mutant could not be resolved by mildly dissociating SDS-PAGE. However we reconstituted the LHC II from delipidated chi b-less mutant thylakoids with the pigment extract derived from wildtype thylakoids. Similar to the native LHC II this reconstituted LHC II bind chi b and transfer light energy from chi b to chi a. 

Sato, N., Sonoike, K., Tsuzuki, M. and Kawaguchi, A. (1995) Impaired photosystem II in a mutant of Chlamydomonas reinhardtii defective in sulfoquinovosyl diacylglycerol. Eur. J. Biochem. 234,16-23. 

Inhibition of Photosynthesis by Substituted 4-Nitrophenols in Wildtype and Five Mutants of Chlamydomonas reinhardtii Thylakoids... 

IN VIVO MANIPULATION OF LIPID COMPOSITION IN MUTANTS OF CHLAMYDOMONAS REINHARDTII ... 

Owers-Narhi L et al. (1979) Reconstitution of cyanobacterial photophosphory-lation by a latent Ca2+-ATPase, Biochem. Biophys. Res. Comm. 90, 1025-1031. Piccioni RG et al. (1981) A nuclear mutant of Chlamydomonas reinhardtii defective in photosynthetic photophosphorylation, Eur. J. Biochem. 117, 93-102. 

Holub, O., Seufferheld, M. J., Gohlke, C., Govindjee, G. J., Heiss, G. J. and Clegg, R. M. (2007). Fluorescence lifetime imaging microscopy of Chlamydomonas reinhardtii Non-photochemical quenching mutants and the effect of photosynthetic inhibitors on the slow chlorophyll fluorescence transients. J. Microsc. 226, 90-120. 

Kunstner P, Guardiola A, Takahashi Y et al. A mutant strain of Chlamydomonas reinhardtii lacking the chloroplast photosystem II psbl gene grows photoautotrophically. J Biol Chem 1995 270 9651-9654. 

De Vitry C, Olive J, Diapier D et al. Posttranslational events leading to the assembly of photosystem II protein complex - a smdy using photosynthesis mutants from Chlamydomonas reinhardtii. ] Cell Biol 1989 109 991-16. 

RECONSTITUTION OF THE LIGHT-HARVESTING CHL a/b PROTEIN COMPLEX OF THE CHL b-LESS MUTANT cbn1-48 OF CHLAMYDOMONAS REINHARDTII WITH A PIGMENT EXTRACT DERIVED FROM WILDTYPE... 

ADAPTATION OF CHLAMYDOMONAS REINHARDTII HIGH CO2-REQUIRING MUTANTS TO LIMITING CO2... 

Reconstitution of the Light-Harvesting CHL alb Protein Complex of the CHL -Less Mutant cbnl-48 of Chlamydomonas reinhardtii with a Pigment Extract Derived from Wildtype 855... 

Adaptation of Chlamydomonas reinhardtii High C02-Requiring Mutants to Limiting CO2 505... 

Dubertret G, Mirshahi A, Mirshahi M, Gerard-Hime C, and Tremolieres A. Evidence from in vivo manipulations of lipid composition in mutants that the t -trans- hexadecenoic acid-containing phosphatidylglycerol is involved in the biogenesis of the light harvesting chlorophyll a/b-protein complex of Chlamydomonas reinhardtii. Eur J Biochem. 1994 226 473-482. 

PS1 The PS 1-prep, introduced in this communication is the first reported with a polyhistidine tag fused to the N-terminus of the PsaF subunit. This construct was possible due to the fact that cyanobacterial PsaF-deletion mutants show no impact on photoautotrophic growth - in contrast to Chlamydomonas reinhardtii, where inactivation of PsaF results in a severe reduction of electron transfer from plastocyanin to PS 1 [Hippier et al. 1997], Also, the N-terminus of the F-subunit which was decorated by the tag is located towards the lumen side which enables an attachment of the isolated PS1 with the lumen-exposed /donor-side to the electrode surface in our hydrogen-producing device. 

Starch is formed in chloroplasts of moss, fern and green algae.18 Chlorophyceae (green algae) starch is similar to that of higher plants, and several species have been used in studies of starch biosynthesis.19,22,29 In a recent set of studies, Ball et al.22 used Chlamydomonas reinhardtii to study starch biosynthesis. They produced several Chlamydomonas mutants which produce starch with characteristics similar to starches produced by maize endosperm mutants.31-34 The various starch mutations of Chlamydomonas will be discussed in Section 3.7. Other classes of algae which produce starch are Prasinophyceae19,35 and Cryptophyceae.35,36... 

Olive, J., Wollman, F.A., Bennoun, P. and Recouvreur, M. 1979. Ultrastruct-ure—function relationship in Chlamydomonas reinhardtii thylakoids, by means of a comparison between the wild type and the F34 mutant which lack the photosystem II reaction center. Molec. Biol. Rep. 5,139-143. 

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