Protein synthesis inhibition mupirocin

The target of mupirocin is one of a group of enzymes which couple amino acids to their respective tRNAs for delivery to the ribosome and incorporation into protein. The particular enzyme inhibited by mupirocin is involved in producing isoleucyl-tRNA. The basis for the inhibition is a structural similarity between one end of the mupirocin molecule and isoleucine. Protein synthesis is halted when the ribosome encounters the isoleucine codon through depletion of the pool of isoleucyl-tRNA. 

Mupirocin is a topical antibiotic that inhibits isoleucyl tRNA synthetase with the subsequent inhibition of protein synthesis. Mupirocin has become a mainstay in the treatment of Staph, aureus infection and colonization during hospital outbreaks, and it is in this organism that acquired resistance has arisen (Gilbart etal. 1993). 

Mupirocin is a naturally occurring antibiotic available as a cream or ointment, and is unusual in its origin and mechanism of action. It inhibits protein synthesis, actively preventing the incorporation... 

Mupirocin is an antibiotic that inhibits bacterial protein synthesis by binding to isoleucyl transfer RNA synthetase. It is primarily bacteriostatic at low concentrations, but is bactericidal in the high concentrations and can have activity against organisms reported to be relatively resistant in vitro. 

Mupirocin is an antibiotic agent that inhibits bacterial protein synthesis. It is indicated in treatment of impetigo caused by Staphylococcus aureus and Streptococcus pyogenes (topical ointment) and treatment of secondarily infected traumatic skin lesions (up to 10 cm in length or 100 cm in area). 

Mupirocin inhibits bacterial protein synthesis by reversible binding and inhibition of isoleucyl transfer-RNA synthetase. There is no cross-resistance with other classes of antibiotics. Low-level resistance, which is not clinically significant, is due to mutations of the host gene encoding isoleucyl transfer-RNA synthetase or an extra chromosomal copy of a gene encoding a modified isoleucyl transfer-RNA synthetase. High-level resistance (MIC > 1 mg/ml) is mediated by a plasmid or chromosomal copy of mupA, which encodes a bypass synthetase that binds mupirocin poorly. 

Mechanisms Mupirocin is a fermentation product of Pseudomonas fluorescens and is unrelated to any other antimicrobial drug. It acts on gram-positive cocci and inhibits protein synthesis by specifically binding to isoleucyl-tRNA synthetase. 

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