Imaging, multispectral

The method of multispectral imaging exploits the characteristics of CCD cameras for obtaining information on the wavelength dependence of the reflectivity of the objects of interest. Different approaches are possible for reaching this goal ... 

Haseloff, J. (1999). GFP variants for multispectral imaging of living ce s. Methods in Cell Biology 58 139-151. 

By analogy, a hyperspectral data set is defined by at least 50 planes an absorbance map is acquired for each wavelength in the spectral range. If the wavelengths number less than 50, the term multispectral imaging is used. 

Tran, C. D. (2003), Infrared multispectral imaging Principles and instrumentation, Appl. Spectrosc. Rev., 38,133-153. 

IR spectroscopy is a fast, simple, and cheap method for the qualitative detection of certain functional groups on insoluble supports [77-79]. Dried supports can be used directly to prepare KBr pellets for standard recording of IR spectra [54,80-82], Newer IR-based techniques, which require much less support material than required for a KBr pellet, include single-bead FT-IR spectroscopy [16,77,83-86], single-bead Raman spectroscopy [87], near-IR multispectral imaging [88], and the simultaneous analysis of several different beads by FT-IR microscopy for analysis of combinatorial libraries [89,90],... 

Tran, C. D., Cui, Y., and Smirnov, S. (1998) Simultaneous multispectral imaging in the visible and near infrared region applications in document authentication and determination of chemical inhomogeneity of copolymers. Anal. Chem. 70, 4701-08. 

Tran, C. D. and Grishko, V. I. (2004) Determination of water contents in leaves by a near-infrared multispectral imaging technique. Microchem. J. 76, 91-94. 

Lu, R. (2004) Multispectral imaging for predicting firmness and soluble solids content of apple fruit. Postharvest Biol. Technol. 31(2), 147-57. 

Methods developed for traditional lossy image compression can be used in the compression of multispectral images. The discrete cosine transform (DCT),... 

Fenniri H, Hedderich HG, Haber KS, Achkar J, Taylor B, Ben-Amotz D, Towards the DRED of resin-supported combinatorial libraries a noninvasive methodology based on bead self-encoding and multispectral imaging, Angew. Chem. Int. Ed., 39 4483-4485, 2000. 

Fischer, M. and Tran, C.D., Investigation of solid phase peptide synthesis by the near-infrared multispectral imaging technique a detection method for combinatorial chemistry, Anal. Chem., 71, 2255-2261, 1999. 

Chourpa I, Lei FH, Dubois P, Manfait M, Sockalingum GD (2008) Intracellular applications of analytical SERS spectroscopy and multispectral imaging. Chem Soc Rev 37 993-1000... 

Rigacci, L., Alterini, R., Bernabei, P.A., Ferrini, P.R., Agati, G., Fusi, F. and Monici, M. (2000) Multispectral imaging autofluorescence microscopy for the analysis oflymph-node tissues. Photochem. Photobiol, 71, 737-742. 

Gittins, C.M. and Marinelli, W., AIRIS multispectral imaging chemical sensor, Electro-Optical Technology for Remote Chemical Detection and Identification lit. Proceedings of SPIE, vol. 3383, 65-74 (1998)... 

NIR imaging experiments create three-dimensional (3-D) images where two dimensions provide spatial information and one dimension provides spectral information. If the third dimension is restricted to a limited number of wavelength bands (typically between 3 and 10), a multispectral image is created consequently, when the whole spectrum is scanned the image created is termed hyperspectral (Figure 8.1). 

Another solution to the problem, based on multispectral imaging, has been developed by Japan Tobacco (Tokyo, Japan) [19], by using three infrared bands in combination with an RGB camera. With this system, 78% of the targeted foreign... 

Figure 8.8 Sorting of tobacco leaves using laser lighting and CCD cameras for creating multispectral images. Illustration courtesy of BEST.

Noordam et al. [53] used a multispectral imager composed of a PbS camera and a filter wheel (680, 740, 800, 840, 1010, lllOnm) for viewing two wooden disks of pine and sprace wood (Figure 8.11). Based on PGA on single pixels, these authors were able to map three regions, namely bark wood, compression wood and normal wood. 

This is typically the case for streams of mixed items that require sorting, an example being in the recycling industries. Several reports [56, 57] and patents [58, 59] are related to on-line hyperspectral or multispectral imaging for the... 

The availability of hyperspectral and multispectral imaging systems with large arrays has brought about new demands in terms of data processing capacity and the mathematics required to extract the important content from these large data sets. As spectral information in images greatly increases the amount of data to be processed, it is absolutely necessary to reduce the dimensionality of the data [66]. 

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