Raman spectroscopy single bead

An important tool for the fast characterization of intermediates and products in solution-phase synthesis are vibrational spectroscopic techniques such as Fourier transform infrared (FTIR) or Raman spectroscopy. These concepts have also been successfully applied to solid-phase organic chemistry. A single bead often suffices to acquire vibrational spectra that allow for qualitative and quantitative analysis of reaction products,3 reaction kinetics,4 or for decoding combinatorial libraries.5... 

Macro-FT Raman Spectroscopy 4c The FT Raman spectra can be acquired in macromode on a small amount of beads. The advantages compared to single-bead Raman measurement are reduced acquisition time and the excitation energy. 

IR spectroscopy is a fast, simple, and cheap method for the qualitative detection of certain functional groups on insoluble supports [77-79]. Dried supports can be used directly to prepare KBr pellets for standard recording of IR spectra [54,80-82], Newer IR-based techniques, which require much less support material than required for a KBr pellet, include single-bead FT-IR spectroscopy [16,77,83-86], single-bead Raman spectroscopy [87], near-IR multispectral imaging [88], and the simultaneous analysis of several different beads by FT-IR microscopy for analysis of combinatorial libraries [89,90],... 

Additionally, Raman spectroscopy as a complement to IR spectroscopy can be applied to resin samples and—using a Raman microscope—to single beads. 

Because of the simple sample preparation, FT-IR spectroscopy [12,13] has become an increasingly popular method in SPOS for reaction monitoring. For IR spectra in the transmission mode [14,15] or with ATR-[16],FT-Raman-[17,18] and DRIFTS-[19] methods, only 1-2 mg of resin beads are required for routine measurements. With FT-IR microscopy [20-24], it is even possible to obtain spectra from single resin beads or spatially resolved spectra [25]. 

---