Multinuclear Preclinical Measurements in Animals

The multi-slice technique of H MRI was used to study regional effects of amphetamine on rat brain [49], a two-coil system enabling three-dimensional perfusion imaging. Amphetamine caused a significant increase in perfusion in many areas of the brain, including the cortex, cingulate and caudate putamen. 

A well-known radiopharmaceutical 2-fluoro-2-deoxy-D-glucose (FDG) is widely used for positron emission tomography (PET) diagnosis of brain activity. It was also evaluated as an NMR pharmaceutical for cancer detection [52]. F chemical shift images of FDG and its metabolites were obtained at 376 MHz, the mice with MH134 hepatoma having been 

Fluorinated representative of antifolates (C2-desamino-C2-methyl-N10-propargyl-2 -trifluoromethyl-5,8-dideazafolic acid), which act as inhibitors of thymidylate synthase and are being evaluated for the treatment of human cancer) was followed in vivo in mice. F images were measured from the abdomen of mice after intravenous injection (500 mg/kg). A time resolution of 4 min was obtained for two-dimensional and 20 min for three-dimensional imaging. The drug was found in gall and urinary bladders [53]. The results confirm the potential value of F MRI in pharmacokinetic studies. 

Fast F MRI with FLASH technique was applied in vivo and the imaging time amounted to only 470 ms [54] this sequence enabled a pharmacological study to be performed on the clearance of perfluorooctylbromide in the internal organs of rats [55]. 

The MRI studies on estradiol-induced pituitary hyperplasia [58] and transplanted Dunning prostate tumors showed that the somatostatin analog Sandostatin significantly reduced the tumor size in rats [59]. 

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