Multilayer coil countercurrent

Vidal, S. et al., Fractionation of grape anthocyanin classes using multilayer coil countercurrent chromatography with step gradient elution. J. Agric. Food Chem. 52, 713, 2004. 

Other methods have also been successfully employed in the isolation procedure of stilbenoids, including MPLC [209,299,326], preparative TLC [208,277,299], centrifugal partition chromatography (CPC) [359,360] and the support-free technique of multilayer coil countercurrent chromatography (MLCCC) [32]. A method based on capillary electrophoresis with electrochemical detection (CE-ED) was employed for the determination of oligomeric stilbenes found in the roots of Caragana species [361]. 

The taste and mouthfeel properties of three different types of tannin-like polyphenolic compounds, representative of some of the tannin-like polyphenolic compounds found in red wines, were determined using descriptive sensory analysis [50]. Ethyl-bridged fiavan-3-ols were produced by reaction of (+)-catechin with acetaldehyde under acidic conditions. Red-colored tannin-like polyphenolic compounds from wine and from wine pomace were isolated by multilayer coil countercurrent chromatography (MLCCC). Mouthfeel attributes and bitterness of the fractions dissolved in a model wine (MW) medium were rated while the fractions were held in the mouth and after expectoration. The sensory properties... 

Fig. 1 Chromatograms of hesperetin (1), kaempferol (2), and quercetin (3) by different countercurrent chromatographic methods. Solvent system chloroform-methanol-water (33 40 27). (A) DCCC (B) RLCC (C) multilayer coil CPC system and (D) cartridge CPC system. (Elsevier Science Ireland, Ltd.)...

Figure 11-14. A multilayer coil-planet countercurrent chromatograph, model DP-950.

Figure 11.13. Design principle of the multilayer coil planet centrifuge for high-speed countercurrent chromatography. (From ref. [235]. John Wiley Sons)...

Hgure 5 Cross-sectional view of seal-free slow rotary countercurrent chromatography (CCC) equipped with a large multilayer coil. 

Source From Improved high-speed countercurrent chromatograph with three multilayer coils connected in series, n. Separation of various biological samples with a semipreparative column in J. Chromatogr. ... 

Countercurrent chromatography (CCC) is a liquid-liquid partition technique that does not involve use of a solid support. In high-speed countercurrent chromatography (HSCCC), one of the two immiscible liquid phases (the stationary phase) is retained in an Ito multilayered coil column by centrifugal force while the other liquid phase (the mobile phase) is pumped through the rotating column. A variation of HSCCC, known as... 

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