Multilayer coil countercurrent chromatography

Vidal, S. et al., Fractionation of grape anthocyanin classes using multilayer coil countercurrent chromatography with step gradient elution. J. Agric. Food Chem. 52, 713, 2004. 

Other methods have also been successfully employed in the isolation procedure of stilbenoids, including MPLC [209,299,326], preparative TLC [208,277,299], centrifugal partition chromatography (CPC) [359,360] and the support-free technique of multilayer coil countercurrent chromatography (MLCCC) [32]. A method based on capillary electrophoresis with electrochemical detection (CE-ED) was employed for the determination of oligomeric stilbenes found in the roots of Caragana species [361]. 

The taste and mouthfeel properties of three different types of tannin-like polyphenolic compounds, representative of some of the tannin-like polyphenolic compounds found in red wines, were determined using descriptive sensory analysis [50]. Ethyl-bridged fiavan-3-ols were produced by reaction of (+)-catechin with acetaldehyde under acidic conditions. Red-colored tannin-like polyphenolic compounds from wine and from wine pomace were isolated by multilayer coil countercurrent chromatography (MLCCC). Mouthfeel attributes and bitterness of the fractions dissolved in a model wine (MW) medium were rated while the fractions were held in the mouth and after expectoration. The sensory properties... 

Figure 11.13. Design principle of the multilayer coil planet centrifuge for high-speed countercurrent chromatography. (From ref. [235]. John Wiley Sons)...

Countercurrent Chromatography Procedure. The entire column (pair of coiled multilayer columns connected in series) was filled with the stationary phase. The apparatus was then rotated counterclockwise at 600 rpm in planetary motion while the mobile phase was pumped into the inlet of the column at a flow-rate of 2.2 mL/min (head to tail elution mode). Maximum pressure at the outlet of the pump measured 80 psi. After a 1-hour equilibration period, the sample was loaded into the Rheodyne injector loop and injected. Effluent from the outlet of the column was continuously monitored with a Shimadzu UVD-114 detector at 312 nm and fractions collected with a Gilson FC-lOO fraction collector to obtain approximately 8.8 mL of eluant in each tube (during a 4-min interval). Retention of the stationary phase was estimated to be 930 mL (74%) by measuring the volume of stationary phase eluted from the column before the effluent changed to mobile phase (330 mL) and subtracting this volume from the total column capacity of 1260 mL. 

Hgure 5 Cross-sectional view of seal-free slow rotary countercurrent chromatography (CCC) equipped with a large multilayer coil. 

Countercurrent chromatography (CCC) is a liquid-liquid partition technique that does not involve use of a solid support. In high-speed countercurrent chromatography (HSCCC), one of the two immiscible liquid phases (the stationary phase) is retained in an Ito multilayered coil column by centrifugal force while the other liquid phase (the mobile phase) is pumped through the rotating column. A variation of HSCCC, known as... 

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