Multifactorial Systematic Method Development and Optimization in Reversed-Phase HPLC

Multifactorial Systematic Method Development and Optimization in Reversed-Phase HPLC 

The idea is to examine the influence of chromatographic factors systematically in real time and to a large extent automatically and to seek out and verify their global optimum , i.e. the optimum constellation. 

This approach is based on publications by Preu etal. [1, 2] on strategy during the optimization of chemical reactions. They pointed out that usually one parameter after another is gradually optimized. A disadvantage of this OVAT (one 

HPLC Made to Measure A Practical Handbook for Optimization. Edited by Stavros Kromidas Copyright 2006 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim ISBN 3-527-31377-X 

Under normal conditions, one systematically changes one factor after another by first trying to separate the sample on a standard stationary phase eluting with an acetonitrile/water mixture, then with a second solvent, such as methanol. 

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Multifactorial Systematic Method Development and Optimization in Reversed-Phase HPLC 623 4.3.5... 

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