Multidimensional Chromatography Biomedical and Pharmaceutical Applications

Other bioanalytical applications of systems in which the eluate of a first LC column is sampled in continuous and repetitive intervals and subjected to a second LC dimension are, for example, described by Wheatly et a/. (11) and Matsuoka et al. (12). Wheatly coupled gradient affinity LC with RPLC for the determination of the isoenzymatic- and subunit composition of glutathione 5-transferses in cytosol 

In biomedical analysis, LC-LC has been used most extensively and successfully in the heart-cut mode for the analysis of drugs and related compounds in matrices Such as plasma, serum or urine. Table 11.1 gives an overview of analytes in biological matrices which have been determined by heart-cut LC-LC systems. A typical example of such an approach is the work of Eklund et al. (16) who determined the free concentration of sameridine, an anaesthetic and analgesic drug, in blood plasma 

Analyte(s) Sample matrix Sample preparation Eirst EC mode Subsequent EC mode(s) Detection Reference 

Drugs and Related Compounds Ampicillin human plasma deproteination C18 C18 post-Elu 28 

Basic drugs human serum liq-liq extraction ion-pair C18 C18 UV 37 

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Somsen, G.W., deJong, G.J. (2002). Multidimensional chromatography biomedical and pharmaceutical applications, in Multidimensional chromatography. Mondello, L., Lewis, A.C., Bartle, K.D., editors. John Wiley Sons, Ltd., New York. 

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