Multi-step screening

A multi-step screening method was employed to isolate and identify a consortium (identified as CDT-4) formed by Pseudomonas and as Nocardia asteroides (ATCC... 

Pharmacophore Models as Part of a Multi-step Screening Approach... 

To our knowledge, there are only few reports on application of screen-printed cholinesterase-based biosensors to food samples. Many other amperometric detection schemes and chemistries have been recently investigated that could enhance the overall analytical performance of screen-printed devices. Nevertheless, the possibility to successfully apply these approaches to real food samples is strictly linked to their ability to surmount the matrix effect that leads either to complicated multi-step sample preparation or to poor recovery. 

Sauton, N., Lagorce, D., Villoutreix, B.O., and Miteva, M.A. (2008) MS-DOCK accurate multiple conformation generator and rigid docking protocol for multi-step virtual ligand screening. BMC Bioinformatics, 9, 184. 

Mercier KA, Shortridge MD, Powers R (2009) A multi-step NMR screen for the identification and evaluation of chemical leads for drug discovery. Comb Chem High Throughput Screen 12(3) 285-295... 

A variety of cationic lipids have been studied as non-viral systems for nucleic acids including DNA and RNA. However, the collection of publicly described lipid materials used for delivery is limited in diversity due in part to their multi-step synthesis. We used high-throughput synthesis and screening techniques to develop a new class of lipid-like materials referred to as lipidoids. Over 1200 unique lipidoid structures were created in this combinatorial library. ... 

On the other hand, the manufacture of the TV screen is a multi-step operation The lithographic procedure has to be repeated for each of the three colours separately and in some cases an extra step is necessary in order to generate a non reflecting matrix pattern around the colour dots The colour dots are produced on the screen by flow... 

Matosevic, S., Lye, G.J., and Baganz, F. (2011) Immobilised enzyme microreactor for screening of multi-step bioconversions characterisation of a de novo transketolase-(o-transaminase pathway to synthesise chiral amino alcohols. J. Biotechnol, 155, 320-329. doi 10.1016/j.jbiotec.2011.07.017... 

Alternatively, Weinmann et al. have used MRM as the survey mode (14), targeting 700 compounds owing to the introduction of the scheduled MRM application (15). However, this latter hybrid technique ( multi-target screening for the first step and full scan identification for the second) cannot be regarded as a general unknown screening, as it can only detect compounds included in a predefined list. 

Quinoline incorporated benzimidazole derivatives were synthesized by Garudachari et al. [18] from substituted aniline and isatin through multi-step reaction. Final compounds were investigated for their in-vitro antimicrobial and antifungal activities by well plate method. Among the screened samples, dichlorosubstituted quinoline derivatives and nitrogene on the bezimidazole moiety showed... 

Another exemplar of the power of multi-step library synthesis is the optimisation of a PDE2 inhibitor HTS hit (Scheme 18.36). The HTS hit itself was derived from a library that was synthesised to enrich the Pfizer screening collection. Key steps in the library synthesis involved reaction of the commercial dichloro pyrazine H with a range of alcohols, followed by reaction of the intermediate chloroalkyoxy pyrazine with hydrazine. Finally, the hydrazine pyridazine I was reacted with a number of aldehydes under oxidative conditions to form the triazolo pyrazine derivatives. This library allowed the project team to find key SAR directions for improvinbg selectivity vs. other PDEs. ... 

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