Molybdenum blue, analytical method

The following procedure has been recommended by the Analytical Methods Committee of the Society for Analytical Chemistry for the determination of small amounts of arsenic in organic matter.20 Organic matter is destroyed by wet oxidation, and the arsenic, after extraction with diethylammonium diethyldithiocarbamate in chloroform, is converted into the arsenomolybdate complex the latter is reduced by means of hydrazinium sulphate to a molybdenum blue complex and determined spectrophotometrically at 840 nm and referred to a calibration graph in the usual manner. 

Matrix effects in the analysis of nutrients in seawater are caused by differences in background electrolyte composition and concentration (salinity) between the standard solutions and samples. This effect causes several methodological difficulties. First, the effect of ionic strength on the kinetics of colorimetric reactions results in color intensity changes with matrix composition and electrolyte concentration. In practice, analytical sensitivity depends upon the actual sample matrix. This effect is most serious in silicate analysis using the molybdenum blue method. Second, matrix differences can also cause refractive index interference in automated continuous flow analysis, the most popular technique for routine nutrient measurement. To deal with these matrix effects, seawater of... 

In an attempt to integrate all the analytical process in a single device, a hyphenated MSFIA-microcolumn semp has been assembled for automated flow-through partitioning and accurate determination of the content of bioavaUable forms of orthophosphate in soils and sediments utilizing the molybdenum blue method for extract processing [103]. 

Phosphate in water may be determined according to a procedure outlined in [9], known as the "molybdenum blue method" It involves the complexation of phosphate with molybdate, with subsequent reduction of the complex with ascorbic acid The result is a complex having an intense blue color The overall reaction rate is limited by the complexation step, with maximum conversion of phosphate to the reduced complex requiring about 10 minutes This analytical procedure has been adapted by many groups for phosphate analysis in flow systems (see, for instance, [2, 10]) In one instance, a system was developed to monitor phosphate concentrations in fermentation broths [11] The flow manifold employed in that application is the model for the phosphate analysis using a stacked system described m this paper... 

Traces of arsenic may be determined either by the molybdenum blue or Gutzeit methods. For determination of arsenic contents from T5 to 15 //g in the sample taken the molybdenum blue method is recommended by the Analytical Methods Committee of the S.A,C. the details are as follows ... 

In the opinion of the Analytical Methods Committee of the S,A,C. the molybdenum-blue method is considered to have inherent advantages over the Gutzeit method, which may be regarded as an estimation rather than a determination, since it depends on the evaluation of the intensity of a stain on a test-paper and the judgments of individual analysts may differ slightly. Nevertheless, within this limitation and provided that the details of the method are strictly observed, the Gutzeit method is adequate in many circumstances, particularly when it is necessary to ascertain whether or not an arsenic content exceeds a certain limit. It is also less intricate and time-consuming than the molybdenum-blue method. 

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