Molisch reagent

Errera examined the distribution of alkaloids in plant tissues by histochemistry and found that alkaloids were present in active tissues near the vegetative points, ovule, epidermis and the layer just inside of it, hair, peripheral layers of fruits and seeds, vascular bundle, cork cambium, cork tissues, and latex tube (9). Molisch microscopically investigated 15 kinds of alkaloids as distinguishable crystal forms after treatment with acids or alkaloid reagents, and then histochem-ically examined them in plant tissue and cell sections following treatment with acids or alkaloid reagents (9). Tunmann and Rosenthaler observed histochemi-cally the distribution of alkaloids in tissues and cells of 36 families of plants 10). 

Naphthol (Molisch s reagent for wool). Dissolve 15 g of 1-naphthol in 100 mL of alcohol or chloroform. 

Reagents x, DiUUe solution of copper, sulphate. 2, Cold nitric acid. 3, saturated solution of sodium chloride + nitric acid. 4, One volume of saturated solution of sodium chloride acidified with acetic acid. 5, Saturation with sodium chloride and acidulation with nitric acid. 6, Neutral solution, saturated sodium chloride. 7, Boiling of the liquid with lead acetate in an alkaline medium. 8, Molische reaction. 

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