Molecular Probes, Inc

Spectra 7-Amino-4-methylcoumarin. Molecular Probes, Inc., http //www.probes.com/servlets/spectra fileid=191 ph7... 

Haugland RP (2002) Handbook of fluorescent probes and research products, 9th edn. Molecular Probes Inc, Eugene... 

Fig. 10.2. Various classes of fluorescent pH indicators. (P-1 to P-8 Haugland R. P., Handbook of Fluorescent Probes and Research Chemicals, 6th edn, Molecular Probes, Inc., Eugene, OR. P-9 de Silva A. P. et al. (1989)...

By keeping the same fluorophores, but reducing the cavity size of the ion-ophore, we obtain PCT-13 (Mag-Indol) and PCT-14 (Mag-Fura2) (Figure 10.22), which are selective for magnesium. PCT-8, PCT-9 and PCT-11 to PCT-14 are commercially available in the non-fluorescent acetoxymethylester form so that they are cell permeant and they recover their fluorescence upon hydrolysis by enzymes (Molecular Probes, Inc., Eugene, OR). 

Haugland R. P., Handbook of Fluorescent Probes and Research Chemicals, 6th edn, Molecular Probes, Inc., Eugene, OR,... 

The increasing interest of researchers for fluorescent probes can be explained by the great improvement of the sensitivity and the spatial or temporal resolution of instruments, and by the development of a wide choice of commercially available probes for particular applications (Molecular Probes, Inc., United States Lambda Fluoreszenztechnologie Ges.m.b.H., Austria). However, there is still a need for probes with improved specific response and minimum perturbation of the microenvironment, in particular in the field of ion recognition which is the object of this chapter. 

Calcium Green (2c), Calcium Orange (2d), and Calcium Crimson (2e) have been developed by Molecular Probes, Inc. they are suitable for fluorescence lifetime imaging of calcium.(71)... 

Haugland RP. 1996. Handbook of fluorescent probes and research chemicals, sixth edition. Eugene, OR Molecular Probes, Inc. 

Dissolve 7-AAD (Molecular Probes Inc.) in buffer at a concentration of 100 pg/ml. The solution should be stored as small aliquots at 4°C or -20° C, and brought to room temperature before use. 

The final concentration of the cRNA transcript can be determined by its absorbance. The absorbance of a 1 1000-2000 dilution of the transcript is read at a wavelength of 260 nm (one unit A260 is equivalent to 40 pg/mL of RNA). Alternatively, densitometry on ethidium bromide-stained agarose gels or colorimetric stains (e.g., RiboGreen, Molecular Probes, Inc., Eugene, OR) can be used to... 

Second, we tried the immobilization of DNA molecules as a potential target material for the immobilization of biological macromolecules. An aqueous solution of 1 mg/mL A.-DNA was spotted onto the surface of an azopolymer and covered with a cover glass, where the A.-DNA was stained with a fluorophore (YOYO-1 iodide, Molecular Probes Inc., Eugene, OR) in advance and the surface was then irradiated with the linearly shaped laser beam for 5 min, as shown in Fig. 3b. The surface was washed for 5 min in an aqueous solution and was then observed using... 

R.P. Haugland, in Handbook of Fluorescence Probes and Research Chemicals , Molecular Probes Inc. 1992. 

However, researchers should be aware that a new noncovalent method for biotinylating antibodies has recently become available. Zenon technology from Molecular Probes, Inc., is a unique immunolabeling system that enables researchers to attach biotin,... 

Research UK, London Research Institute Laboratories, London, UK Rosaria P. Haugland Molecular Probes Inc., Eugene, OR James D. Hirsch Molecular Probes Inc., Eugene, OR David Hughes Nuffield Department of Surgery, John Radcliffe Hospital, Oxford, UK... 

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