Molecular diagnostics nucleic acid extraction

The specimen will be the basis for the analytic analysis. Is it RNA or DNA What is the origin of the tissue Amniocentesis Was it a spontaneous product of conception Were anatomic pathology slides or tissue blocks prepared Are cell lines involved Are these primary or immortalized Was a chorionic villus sampling procedure done Is the sample properly collected peripheral blood The answers to each of these questions should be noted, and considered part of the validation of a useful nucleic acid extraction method. A molecular diagnostics laboratory should adhere to the highest standards in providing services, and prior validation of applicable nucleic acid extraction procedures is a must to ensure high-quality service. 

Recently, the use of AR has extended into several other areas, yielding interesting information for cytology, fresh cell/tissue sections, and fluorescence IHC (fluorescence in situ hybridization [FISH]), in addition to adaptations of the method for extraction of nucleic acids and proteins from FFPE tissues for use with modern methods of molecular analysis. In this chapter, the emphasis is on expanded applications in diagnostic cytology, fresh frozen cell/... 

This chapter illustrates the characterizations of technical procedures necessary in the course of these molecular evaluations, describing our laboratory experience and suggesting different diagnostic approaches microdissection techniques, extraction of nucleic acids from paraffin-embedded tissues, polymerase chain reaction (PCR), direct sequencing, and allelic separation by cloning [4-7],... 

The specific extraction of nucleic acid molecules is the only method of molecular biology-based diagnostic. Jiang et al. have reported the isolation of messenger ribonucleic acid (mRNA)... 

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