Mixed-culture method

Mildew resistance of plastics, mixed culture method, agar medium 6091 ... 

We also conducted experiments to compare our culture method with the standard neurosphere culture. In the standard neurosphere culture, cell number increased approximately nine times over 5 days. Immunostaining showed that the neurosphere cultures contained 54 5.3% nestin+ cells and 41 7.4% nestin+ pIIF cells. This demonstrated that the standard neurosphere culmring method was less efficient than EGF-immobilized substrates for selectively expanding NSCs. Thus, the EGF-immobilized substrates prepared from mixed SAMs with 10% COOH-thiol provided the most efficient method for selective NSC expansion. 

In order to obtain pure cultures, the crude culture which contained Chromatium species was serially diluted in this modified medium as suggested by van Niel ( 1). This was repeated several times to ensure the purity of the culture. The agar shake method was not used as the large photosynthetic bacteria fail to grow in agar shake tubes ( 1). In pure culture the Chromatium species did not grow as well as in the mixed culture situation. The culture was not as dense or as rich in colour as the crude cultures. 

The inocula totalled 1 ml in each case, the mixed culture having 0.5 ml inocula of each of the two organisms. For each experiment, a bottle was taken of each culture and the sulfide and dry weight measured. Sulfide was determined by the method of Pachmayr as described by Triiper and Schlegel (11). The concentration of sulfide was measured by absorbance using a Beckman DB spectrophotometer at 670 nm, and a standardized calibration curve. 

The present study describes a new method for culturing the heart urchin E. cordatum under controlled laboratory conditions. Juveniles fed with algae layer of mixed cultures C. calcitrans,... 

None of the pure cultures that produced HFBT have been shown to further metabolize this compound. Bohonos et al. (46) found two further oxidation products, 3-hydroxybenzothiophene and 2,3-dihydrobenzothiophene-2,3-dione in aerobic mixed cultures co-metabolizing dibenzothiophene. Recently, Mormile and Atlas (61) inoculated portions of the filter-sterilized supernatant from a dibenzothiophene-degrading culture with soil and sediment samples and observed the loss of HFBT using a spectroscopic method. Under their aerobic growth conditions, they also observed the release of carbon dioxide from these cultures indicating that these products of dibenzothiophene degradation can be further oxidized. In addition, they observed carbon dioxide production from dibenzothiophene-sulfoxide. 

Islam, S.M., Fukushi, K., Yamamoto, K. (2005). Development of an enumeration method for arsenic methylating bacteria from mixed culture samples. Biotechnol. Lett. 27 1885-90. 

Pure culture yeasts are available on slants or in lyophilized form. They may be propagated in wineries without complete asepsis (26,27), since the low pH of grape must inhibits many bacteria wine fermentations are mixed cultures naturally. Production of active dry wine yeasts (WADY) began in the 1960s. These are produced by bakers yeast companies and are grown by methods resembling those used for bakers yeast production, described eadier. 

Karmali MA, Petrie M, Lim C, Cheung R, Arbus GS (1985) Sensitive method for detecting low numbers of verotoxin-producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin. J Clin Microbiol 22 614-619... 

C has been used as a tracer in the study of the degradation of [1-13C]-acenaphthene both in pure cultures that were degrading naphthalene and phenanthrene by cometabolism, and in a mixed culture that was enriched with creosote (Selifonov et al. 1998). The degradation pathway that is initiated by benzylic monooxygenation could be determined by isolation of intermediate metabolites, and the method proved applicable to the situation where only limited biotransformation of the substrates takes place by partial oxidation. 

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