Mitogen-activated protein kinase activity detection

Like the other two a2-AR subtypes, the site for polymorphic variation of the a2C-AR is localized to the third intracellular loop (1) (Fig. 1). For this subtype, a 12-nucleotide in-frame deletion resulting in a four-amino acid (Gly-Ala-Gly-Pro) loss has been detected, primarily in subjects of African descent (Table 1). In CHO cells, the a2C-Del322-325 displays markedly depressed function as assessed by inhibition of adenylyl cyclase (Fig. 10), stimulation of mitogen-activated protein kinase p44/42, and stimulation of inositol phosphates (1). The basis for this impairment is not clear. Although the third intracellular loop is... 

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