Metal-enhanced fluorescence

Aslan, K., Leonenko, Z., Lakowicz, J.R. and Geddes, C.D. (2005) Fast and slow deposition of silver nanorods on planar surfaces Application to metal-enhanced fluorescence. The Journal of Physical Chemistry. B, 109, 3157-3162. 

Aslan K, Gryczynski I, Malicka J, Matveeva E, Lakowicz JR, Geddes CD (2005) Metal-enhanced fluorescence an emerging tool in biotechnology. Curr Opin Biotechnol 16 55-62... 

Lakowicz JR (2005) Radiative decay engineering 5 metal-enhanced fluorescence and plasmon emission. Anal Biochem 337 171-94... 

Aslan K, Wu M, Lakowicz JR, Geddes CD (2007) Fluorescent core-shell Ag Si02 nanocomposites for metal-enhanced fluorescence and single nanoparticle sensing platforms. J Am Chem Soc 129 1524-1525... 

In addition to the application of roughened Ag electrodes in SERS measurements, one should add that Geddes et al. [15] have described the use of such Ag electrodes in metal-enhanced fluorescence studies also. The constant current flowing between two silver electrodes in pure water facilitated the growth of fractal-like structures on the cathode. The electrode was coated with a monolayer of human serum albumin protein labeled with Indocyanine Green. It was observed that the fluorescence intensity on the roughened electrode increased approximately the 50-fold, compared to the unroughened electrode. 

Aslan K, Holley P, Geddes CD (2006) Microwave-accelerated metal-enhanced fluorescence (MAMEF) with silver colloids in 96-well plates Application to ultra fast and sensitive immunoassays, high throughput screening and dmg discovery. J Immunol Methods 312 137-147 Matveeva E, Gryczynski Z, Malicka J et al (2004) Metal-enhanced fluorescence immunoassays using total internal reflection and silver-coated surfaces. Anal Biochem 334 303-311 Blue R, Kent N, Polerecky L (2005) Platform for enhanced detection efficiency in luminescent-based sensors. Electron Lett 41 682-684... 

Metal-enhanced fluorescence / edited by Chris D. Geddes. p. cm. 

In about 2000, my laboratory started to study the interactions of fluorophores with metallic nanoparticles, both solution-based and surface-immobilized. Our findings agreed with other workers whom had observed increases in fluorescence emission coupled with a decrease in the fluorophores radiative lifetime. Subsequently, we applied classical far-field fluorescence descriptions to these experimental observations, which ultimately suggested a modification in the fluorophores s intrinsic radiative decay rate, a rate thought to be mostly unchanged and only weakly dependent on external environmental factors. This simple description, coupled with what seemed like a limitless amount of applications led to a paper published by our laboratory in 2001 entitled Metal-Enhanced Fluorescence , or MEF, a term now widely used today almost a decade later. 

Metal-Enhanced Fluorescence Progress Towards a Unified Plasmon-Fluorophore Description... 

Figure 1.2. (A) A schematic diagram depicting the processes in close proximity to metals (< 10 nm) involved in Metal-Enhanced Fluorescence enhanced absorption and coupling to surface piasmons. (B) Emission spectra of FITC deposited onto SIFs and glass. The inset shows the real-color photographs of FITC emission from these surfaces. (C) Intensity decays for FITC on both glass and SiFs. IRF Instrument Response Function.

K. Aslan, M. J. R. Previte, Y. X. Zhang, and C. D. Geddes. Metal-enhanced fluorescence (MEF) Progress towards a unified plasmon-fluorophore theory Biophysical journal, 2007, 371A-371A. 

K. Aslan, Z. Leonenko, J. R. Lakowicz, and C. D. Geddes. Annealed silver-island films for applications in metal-enhanced fluorescence Interpretation in terms of radiating plasmons yourna/ of fluorescence, 2005, 15, 643-654. 

K. Aslan, S. N. Malyn, and C. D. Geddes. Angular-dependent metal-enhanced fluorescence from silver colloid-deposited films opportunity for angular-ratiometric surface assays The Analyst, 2007,132,1112-1121. 

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