Microstructure studies confocal laser scanning microscopy

Falke K, Buttner A, Schittkowski M, Stacks O, Kraak R, Zhivov A, Rolfs A, Guthoff R. The microstructure of cornea verticillata in Fabry disease and amiodarone-induced keratopathy a confocal laser-scanning microscopy study. Graefes Arch Chn Exp Ophthalmol 2009 247 523-34. 

With the advent of confocal laser scanning fluorescence microscopy (CLSFM) [39], multiple photon microscopy (MPM) [40,41], and atomic force microscopy (AFM) [5], three new tools have been added to the standard tools of light microscopy (LM) [42-45] and electron microscopy (EM) [35,46-50] that were most widely used in the past to study the microstructure of fats and foods in general. The work of Heertje et al. [35,36,39,51] on visualization of the microstructure in fats remains one of the most important contributions to the field. In their method, a cold solvent mixture (butanol-methanol) was used to remove the liquid oil form the solid fat in a sample mounted on a special holder. After removal of the liquid oil, the structure of the solid fat network could be visualized. 

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