Micromachined Flow Cytometer

S. Gawad, K. Cheung, U. Seger, A. Bertsch and Ph. Renaud, Dielectric spectroscopy in a micromachined flow cytometer theoretical and practical considerations, Lab Chip, 4, 241-251 (2004). 

Microfabricated Coulter counter Microfabricated flow cytometer, Micromachined Coulter counter Micromachined flow cytometer. Miniaturized fluorescence-activated cell sorting devices... 

Over the past decade, the drawbacks of conventional flow cytometers coupled with the increased need for miniaturization and sophisticated analysis have motivated the development of the micromachined flow cytometer. It has many potential benefits including disposability, smaller size, lower consumption of sample. 

Lee and coworkers (2006) presented a compact cell counting/sorting system with several essential components including a micromachined flow cytometer chip device, an optical detection system, and a data analysis and control system to achieve the functions of cell sample injection, optical signal detection, and cell collection. Its dimensions were 37 cm in length, 16 cm in width, and 18 cm in height (Fig. 8c) [16]. 

Lee GB, Hung Cl, Ke BJ, Huang GR, Hwei BH, Lai HF (2001) Hydrodynamic focusing for a micromachined flow cytometer. Trans ASME J Fluids Eng 123 673-679... 

Micromachined flow cytometers open new opportunities to integrate optical components and sohd-state devices... 

S. Gaward, L. Schild, and P. Renaud, Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing. Lab on a Chip 1, 76-82 (2001). 

We briefly describe the general methods to fabricate flow cytometers. Conventional flow cytometers contain flow chambers or cuvettes made from glass or quartz tubes, with an injector inserted into the middle of the tube. Recently, microfabricated flow cytometers have been developed using photolithography and micromachining techniques. The two kinds of micromachining... 

Flow Cytometer Lab-on-a-Chip Devices, Fig. 6 Micromachined impedance flow cytometer (a) side schematic view of the microchannel showing a particle passing over three electrodes (A, B, and C). The impedance signal is measured differently (Zac - Zbc). As the distance between two measurement areas and time separating the signal spikes are known, the speed of the particle can be calculated, and (b) signal in-phase amplitude of 2,000 erythrocytes and ghost cells recorded simultaneously for two frequencies [9]... 

Gawad S, Schild L, Renaud P (2001) Micromachined impedance Spectroscopy flow cytometer for cell analysis and particlesizing. Lab Chip 1 76-82... 

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