Microinjection Xenopus embryo

The mechanisms that regulate muscle differentiation in mammals and neural differentiation in Drosophila (and probably mammals as well) bear remarkable similarities. What proteins function analogous to MyoD, myogenin. Id, and E2A in neural cell differentiation in Drosophila Based on these analogies, predict the effect of microinjection of MyoD mRNA on the development of Xenopus embryos. 

However, there are other reasons for working on Xenopus. The animal is completely aquatic, and therefore much easier to keep than other amphibians, which have a tendency to hop away. It is also rather robust, and only rarely succumbs to disease or infection. The early embryo is relatively large in size and, like all amphibian embryos, is readily accessible to the investigator because it develops outside the mother. Dissection and microinjection are therefore easily performed, and because each embryonic cell is provided with yolk reserves to serve as an energy source, tissues can be isolated and cultured for several days in simple salt solutions without the need for poorly characterized serum components. Finally, development is rapid. Together, these virtues have made the Xenopus embryo a favorite of vertebrate developmental biologists. 

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