Microdialysis methods

Baumann, M.H. and Rutter, J.J., Application of in vivo microdialysis methods to the study of psychomotor stimulant drugs, in Methods in Drug Abuse Research, Cellular and Circuit Level Analysis, Warerhouse, B.D., Ed., CRC Press, Boca Raton, FL, 2003, 51-86. 

Parent M, Bush D, Rauw G, Master S, Vaccarino F, et al. 2001. Analysis of amino acids and catecholamines, 5-hydroytryp-tamine and their metabolites in brain areas in the rat using in vivo microdialysis. Methods 23 11-20. 

Khan SH, Shuaib A. 2001. The technique of intracerebral microdialysis. Methods 23(1) 3-9. 

Lonnroth P, Jansson PA, Smith U. 1987. A microdialysis method allowing characterization of intercellular water space in humans. Am J Physiol 253(2 Pt 1) E228-E231. 

The recently developed microdialysis method [3], which is a technique to sample the extracellular space, was used to study the effects of the dopamine... 

Huinink KD, Korf J. Ultraslow microfiltration and microdialysis for in vivo sampling principle, techniques, and applications. In WesterinkBHC, Cremers TIFH (Eds), Handbook of Microdialysis Methods, Applications and Clinical Aspects. Elsevier, Amsterdam, 2007. 

Westerink BHC, Cremers TIFH. Handbook of Microdialysis Methods, Applications and Clinical Aspects, Elsevier, Amsterdam, 2007. 

RosdahlH, Hamrin K, Ungerstedt U, Henriksson J. A microdialysis method for the in situ investigation of the action of large peptide molecules in human skeletal muscle detection of local metabolic effects of insulin. International Journal of Biological Macromolecules 2000, 28, 69-73. 

Oxygen free radicals are elaborated during ischemia and reperfusion and have been strongly implicated in the pathophysiology of ischemic brain injury (114). These reactions may lead to oxidative injury to cellular lipids, proteins, and nucleic acids. Evidence for free radical elaboration in ischemia is obtainable by means of a microdialysis method in which administered salicylate is converted, in the presence of hydroxyl radical, to dihydroxybenzoic acid (DHBA) species—stable adducts detectable by chromatographic methods. A study from our laboratory of... 

Sjbberg P. Olofsson IM, Lundqvist T (1992) Validation of different microdialysis methods for the determination of unbound steady-state concentrations of theophylline in blood and brain tissue. Pharm Res 9 1592-1598. 

The possibility of monitoring changes in subcutaneous glucose concentration by the microdialysis method was first demonstrated by Lonnroth [98]. Other reports followed [99-104], but in all of these, measurements were made in a discontinuous way using traditional techniques. 

Somewhat different results were reported from other laboratories n-3 fatty acid deficiency in rats caused a decrease in dopamine level and D2-receptor density in the frontal cortex as well as a decrease in serotonin receptor density (Delion, 1994,1996), whereas fish oil increased the dopamine level (Chalon, 1998). A decrease in the dopamine release from the frontal cortex was also detected by the microdialysis method after thyramine stimulation but not after KCl stimulation (Zimmer, 1998, 2000). In aged rats, however, the monoamine level was not affected, whereas monoamine oxidase activity was decreased during n-3 fatty acid deficiency (Delion, 1997). 

Winter C D, lannotti E, Pringle A K, et al. (2002). A microdialysis method for the recovery of IL-lbeta, IL-6 and nerve growth factor from human brain in vivo. /. Neurosci. Meth. 119 45-50. 

A variety of techniques are available for protein binding measurement including equilibrium dialysis, ultrafiltration, spectrofluorometric, and microdialysis methods. 

Takeda S, Sato N, Ikimura K, Nishino H, Rakugi H, Morishita R. Novel microdialysis method to assess neuropeptides and large molecules in free-moving mouse. Neurosci 2011 186 110-9. 

Crystallization was performed using a microdialysis method [14] on 40 microliters samples, at constant p-octylglucoside concentration ( 8 mg/ml) using PEG 4000 and NaCI as precipitants. We already reported that slow oxidation of the bacteriochlorophyll dimer occurred when crystallization was performed under air atmosphere [15]. This oxidation was minimized when PEG was scrubbed of peroxides according to [16] and when crystallization was done in air-tight vials, flushed with argon prior to the experiments [15]. 

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