DNA adduct detection

Figure 3. Structure of major DNA adduct detected in many in vivo systems as a result of metabolic activation of benzo[a]pyrene or the reaction of anti-B[alPDE with DNA jji vitro dR=deoxyribose moiety.

Histologic and skeletal abnormalities in all BaP-exposed groups depressed mitotic rates in retina and brain in groups exposed to 0.08 pg/L and higher muscle necrosis in all groups exposed to 0.2 pg/L and higher microphthalmia was observed in 17% of BaP-treated trout No DNA adducts detected in liver... 

Subadults fed diets for 6 days containing either 58 or 288 pg BaP daily, equivalent to 43 mg/kg BW or 216 mg/kg BW Liver DNA adducts detected in both cases 19... 

Halbrook RS. Kirkpatrick RL, Bevan DR. 1992. DNA adducts detected in muskrats by P-postlabeling analysis. Environ Toxicol Chem 11 1605-1613. 

Vahakangas K, Trivers G, Rowe M. et al. 1985. Benzo[a]pyrene diolepoxide-DNA adducts detected by synchronous fluorescence spectrophotometry. Environ Health Perspect 62 101-104. 

Randerath, K., and Randerath, E. (1994). 32P-postlabeling methods for DNA adduct detection Overview and critical evaluation. Drug Metab Rev 26, 67-85. 

Singh, R., and Farmer, P. B. (2006). Liquid chromatography-electrospray ionization-mass spectrometry The future of DNA adduct detection. Carcinogenesis 27, 178-196. 

Whong, W. Z., Stewart, J. D., and Ong, X (1992). Comparison of DNA adduct detection between two enhancement methods of the 32P-postlabelling assay in rat lung cells. Mutat Res 283, 1-6. 

Endo Y, Hara I. 1991. DNA-adduct detection in rats administered with 4,4 -methylenedianiline or 4,4 -methylenebis(2-chloroaniline). J Ind Health 33 430-431. 

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