Microbiological tests oxidation

Microbiological Associates Inc. 1996. Pentabromodiphenyloxide and octabromodiphenyl oxide. Maximization test in Guinea Pigs. Submitted to U.S. EPA under TSCA Section FYI, Fiche no. OTS0001281. 

Prior to 1941, mj/o-inositol was often determined by isolation, as such or as the hexaacetate.87 The isolated inositol was weighed, or was oxidized to carbon dioxide (which was measured in a gas buret).92 In 1941, Woolley98 published his microbiological method, in which the yeast Saccharomyces cerevisiae was used as a test organism. Soon afterward, there appeared an improved procedure, using Saccharomyces carlsbergensis,9i 96 and an additional method based on the discovery of an inositol-less mutant of the common bread-mold, Neurospora crassa.96-98 Plundreds of types of foods,... 

Glycerol 85 % is a less potent preservative than propylene glycol. It acts as a preservative above 30 %. In lower concentrations it is less effective. Microbiological challenge tests of a zinc oxide cutaneous suspension for example showed an insufficient effect in a concentration of 110 mg/g Glycerol 85 %. Therefore it was replaced with propylene... 

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