Microbiological problem areas

Recent interlaboratory comparisons of HPLC and microbiological methods for vitamin B6 revealed significant variability among laboratories (42,70). The extraction and hydrolysis of the B6 vitamers, especially the pyridoxine-/ -glucoside (PNG) in plant-based foods, were cited as problem areas. Other sources of analytical error included HPLC (mis)identification of the individual B6 vitamers, and vitamer interconversion during extraction and analysis. 

The fact is that despite there being myriad microorganisms (and a range of resultant, potentially serious, microbiologically induced problems), we are, for the most part, primarily concerned with only three problem areas. These areas are all controllable and represent microbiological problems where... 

Lundgren, D.G., 1975. Microbiological problems in strip mine areas relationship to the metabolism of Thiobacillus ferrooxidans. Ohio J. Sci., 75 280—287. 

Once biological activity has been established (in the laboratory or the field) and once the chemical work has been accomplished, we need to confirm allelopathic activity in the natural environment. To accomplish this end, the effects of soil and microbial flora must be considered. Thus, the disciplines of soil chemistry and microbiology are required. The chapters in this volume deal primarily with the biology and chemistry of phytotoxins isolated from plants however, we hope that these topics will stimulate soil chemists and microbiologists to contribute to solving the problems associated with the study of allelopathy. Thus, the purpose of this volume is not only to bring before the scientific community a representation of research efforts in the area of allelopathy, but also to promote the relationships... 

HPhe irradiation of fishery products covers a broad area and includes microbiological, biochemical, organoleptic, and engineering problems in the field of fishery technology. Many advances have been made in recent years in the radiation pasteurization of fish and shellfish. 

The economic interest is very important in the development of the drugs. The separation methods are more economical than the asymmetric synthetic methods, and hence the development of rapid, reproducible, and inexpensive resolution methods is a demanding area. Conventionally, enantiomeric separation is performed by fractional crystallization, microbiological methods, kinetic enzymatic resolution, or by chromatographies [Ilisz et al., 2006), which have the problems of robustness, reproducibility, ease, and cost effectiveness. 

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