Microbial management for bacterial pathogen control in invertebrate aquaculture hatcheries

Microbial management for bacterial pathogen control in invertebrate aquaculture hatcheries 

E Goulden, L. H0j and M. R. Hall, Australian Institute of Marine Science (AIMS), Australia 

Key words hatcheries, microbial management, pathogens, probiotics, quorum sensing. 

2 Methods to study bacterial communities in hatchery systems 

Several recent studies have demonstrated the importance of understanding the general microbial communities of which aquaculture pathogens are members. For these studies, the culture-based approaches have severe limitations since only 0.01-10 % of bacteria in marine systems can be cultured by conventional techniques (Connon and Giovannini, 2002 Osborne and Smith, 2005). In addition, the appropriateness of the culture media used and culture conditions will affect the fraction of this culturable community that is successfully recovered. For instance, many studies of fish gastrointestinal microbiota have not included anaerobic culture conditions and have therefore missed certain fastidious and obligate anaerobes completely (Nayak, 

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