Micro-spectroscopic systems

Construction of Micro-Spectroscopic Systems and their Application to the Detection of Molecular Dynamics in a Small Domain... 

Analytical interfaces are integrated into the AuMpRes set-up for at-line analysis by sampling and subsequent chromatography (HPLC) [108], Moreover, this allows online analysis by infrared or Raman spectroscopy. Real-time monitoring of the chemical processes can be achieved via spectroscopic measurements, for which suitable optical flow-through cells have to be installed at selected positions of the micro reaction system. 

Using a thermal micro ATR/ET IR spectroscopic system, a quantitative investigation was made of the molecular interactions in aqueous solutions of poly(A-isopropylacrylamide) (PNIPAAM). The results indicated that intermolecular interactions predominate between PNIPAAM and water at temperatures below the lower critical solution temperature (LOST). However, above the LCST, PNIPAAM molecules in water are aggregated because of intramolecular interactions and hydrophobic interactions in the system (93). 

No.l0, 1999,p.2619-24 THERMAL MICRO ATR/FT-IR SPECTROSCOPIC SYSTEM FOR QUANTITATIVE STUDY OF THE MOLECULAR STRUCTURE OF POLY(N-ISOPROPYLACRYLAMIDE) IN WATER Shan-Yang Lin Ko-Shao Chen Liang Run-Chu Taipei,Veterans General Hospital Tatung,Institute of Technology... 

Lin, S. Y Chen, K. S. Liang, R. C. Thermal micro ATR/FT-IR spectroscopic system for quantitative study of the molecular structure of poly(N-isopropylacrylamide) in water. Polymer 1999, 40, 2619-2624. 

Two major fillers used in the rubber industry are silica and carbon black. Carbon black is black because it absorbs/scatters all radiation, including infrared, impinging on it. Hence, simple transmission spectroscopy of carbon black filled specimens is not straightforward and is usually not possible unless the samples are very thin. Carbon black filled samples have not been readily examinable using micro-spectroscopic methods. Silica filled systems are more amenable to microscopic techniques [76] and can be examined to determine silica-polymer(rubber) interactions. The presence of inorganic materials, e.g., transition metal complexes in... 

Conclusive evidence is produced by infrared or mass spectroscopic methods. This involves the selective trapping of each product as it is eluted from the column. Efficient trapping is obtained by quenching in two or more U-tubes containing glass wool followed by trapping in a breakseal trap for subsequent analysis (Fig. 56). Flow may be diverted to this trapping system by a 3-way solenoid valve operated by a micro-switch. The latter is activated by the pen of the recorder incorporated in the glc when the peak due to the required material reaches 80 % of its maximum. This system has also been used on a small preparative scale. 

Two methods are used to capture proteomes 2D electrophoresis (Section 7.3) and the SELDI protein chip system (Section 7.5). These methods are complemented by the good old micro-sequencing (Section 7.6) and different mass spectroscopic methods (Sections 7.4 and 7.6.5). After all, via partial sequences and the exact MW the proteins of a spectrum can be unambiguously identified in databases. 

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