Methylotrophic Yeasts

In nature, methanol is produced either by oxidation of methane (e.g., by methano-oxidizing bacteria in the rhizosphere of plants) or by the decomposition of pectin and hgnin (which contain high amounts of methyl ester and methoxyl groups) during growth or decay of plants [4, 5]. 

---

This study reports on the production of pectic enzymes and partial characterization of polygalacturonases produced by Candida boidinii. Candida boidinii belongs to the so called methylotrophic yeasts with famous utilization of methanol. Pectin, the natural substrate of pectic enzymes complex, can serve for microorganisms as a C - source by two different ways after deesterification with pectinesterase as methanol and after hydrolytic cleavage with... 

DuPont has developed a process for the manufacture of glyoxylic acid by aerobic oxidation of glycolic acid (Fig. 2.33) mediated by whole cells of a recombinant methylotrophic yeast (Gavagnan et al, 1995). The glycolic acid raw material is readily available from the acid-catalysed carbonylation of formaldehyde. Traditionally, glyoxylic acid was produced by nitric acid oxidation of acetaldehyde or glyoxal, processes with high E factors, and more recently by ozonolysis of maleic anhydride. 

Trimble, R.B., Atkinson, P.H., Tschopp, J.F. et al. (1991) Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the methylotrophic yeast Pichia pastoris. The Journal of Biological Chemistry, 266 (34), 22807-22817. 

Cereghino, J.L. and Cregg, J.M. (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiology Reviews, 24 (1), 45-66. 

Lin-Cereghino, J., Wong, W.W., Xiong, S. et al. (2005) Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris. Biotechniques, 38 (1), 44, 46, 48. 

Clark, D.S., Geresh, S. and DiCosimo, R. (1995) Enantioselective oxidation of 2-methyl-1-alkanols by alcohol oxidase from methylotrophic yeasts. Bioorganic Medicinal Chemistry Letters, 5 (13), 1383-1388. 

Methylophilus methylotrophus, 11 3 Methylotrophic yeasts, 72 479 Methylparaben, antimicrobial used in cosmetics, 7 831t Methyl Parathion... 

The enantioselective hydrolysis of the key industrial starting compound methyl benzoate 41 was recently reported using a Candida rugosa isoenzyme LIP1 expressed in the methylotrophic yeast Pichia pastoris affording /-menthol 28 with > 99% ee. 69... 

Niu et al. (2008) used the methylotrophic yeast P. pastoris vector pPICZa-A in which transcription of the target protein is controlled by the very... 

Korpan YI, Gonchar MV, Starodub NF, et al. 1993. A cell biosensor specific for formaldehyde based on pH-sensitive transistors coupled to methylotrophic yeast cells with genetically adjusted metabolism. Anal Biochem 215 216-222. 

In methylotrophic yeast with peroxisomal damage induced by peroxisomicine A, A2 and tullidinol no evidence of catalase inhibition was seen, either. Considering these results, it has been suggested that catalase is not directly involved in the selective effect of these compounds on peroxisomes. 

Gellissen G (2000). Heterologous protein production in methylotrophic yeasts. Appl. Microbiol. Biotechnol. 54 741-750. 

Gellissen G, Weydemann U, Strasser A W M, Piontek M, Janowicz Z A, Hollenberg C P (1992). Progress in developing methylotrophic yeasts as expression systems. Trends Biotechnol. 10 413-417. 

---