Methanotrophic Methylosinus trichosporium

The methanotroph Methylosinus trichosporium strain OB3b that produces the soluble MMO system consisting of a 40 kDa NADH oxidoreductase, a 245 kDa hydroxylase, and a 16 kDa protein termed component B has a low substrate specificity (Sullivan et al. 1998). 

Sullivan JP, D Dickinson, HA Chase (1998) Methanotrophs, Methylosinus trichosporium OB3b, sMMO, and their application to bioremediation. Crit Rev Microbiol 24 335-373. 

Lindner A. S., Whitfield C., Chen N., Semrau J. D., and Adriaens P. (2(X)3) C iantitative stmcture-biodegradation relationships for orr/io-substituted biphenyl compounds with whole-cell methanotroph, Methylosinus trichosporium OB3b. Environ. Toxicol. Chem. (in press). 

Fox, B. G. Froland, W. A. Dege, J. E. Lipscomb, J. D. Methane monooxygenase from Methylosinus trichosporium OB3b. Purification and properties of a three-component system with high specific activity from a type II methanotroph. J. Biol. Chem. 1989, 264(17), 10023-10033. 

Similar forms of sMMO are expressed by morphologically and meta-bolically distinct methanotrophs that have been classified as Type X and Type II (Lipscomb, 1994). These are typified by Methylococcus capsulatus Bath (MMO Bath) and Methylosinus trichosporium OB3b (MMO OB3b), respectively. The parallel studies of sMMO from these two organisms has facilitated the description of both the essential features of the enzyme... 

In Methylosinus trichosporium OB3b a type II methanotroph, the 50-mer amplified product could readily be identified after the samples had been purified by standard procedures before analysis. Methy-lomicrobium albus BG8, a type I methanotroph with a 99-mer could also be identified, and no cross-amplification products were observed. The application of this procedure is noted in Sections 5.8 and in 8.1.2. 

Xin JY, Zhang YX, Zhang S, Xia CG, Li SB. Methanol production from CO(2) by resting cells of the methanotrophic bacterium Methylosinus trichosporium IMV 3011.J Basic Microbiol 2007 47 426-35. Ashok S, Raj SM, Rathnasingh C, Park S. Development of recombinant Klebsiella pneumoniae dhaT strain for the co-production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol. Appl Microbiol Biotechnol 2011 90 1253-65. 

Xin JY, Zhang YX, Zhang S, Xia CG, Li SB. Methanol production from CO(2) by resting cells of the methanotrophic bacterium Methylosinus trichosporium IMV 3011.J Basic Microbiol 2007 47 426-35. 

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