Metanephrine determination

Peterson ZD, Collins DC, BowerbankCR, Lee ML, Graves SW. 2002. Determination of catecholamines and metanephrines in urine by capillary electrophoresis-electrospray ioniza-tion-time-of-flight mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 776 221. 

A sensitive spectrophotometric method based on the strong absorption of the aminochrome-sodium bisulfite addition products (see Section IV, F) at ca. 350 m/x. has been described recently by van Espen128and Oesterling and Tse 277-278 for determining total catecholamines. While not as sensitive as the fluorimetric procedures, this method is considerably more sensitive than the older colorimetric methods based on the visible absorption peak of the aminochromes. Also, it does not have many of the disadvantages (e.g. costly equipment and unstable blanks) often associated with fluorimetric techniques. The basic procedure can be satisfactorily applied to the differential determination of mixtures of adrenaline, noradrenaline, dopamine, metanephrine, and normetanephrine.178... 

Gamache, P. H., Kingery, M. L., and Acworth, I. N. (1993). Urinary metanephrine and norme-tanephrine determined without extraction by using hquid chromatography and coulometric array detection. Clin. Chem. 39 1825-1830. 

Lenders JW, Eisenhofer G, Armando I, Keiser HR, Goldstein DS, Kopin IJ. Determination of metanephrines in plasma by liquid chromatography with electrochemical detection. CHn Chem 1993 39 97-103. 

Marini M, Fathi M, Vallotton M. [Determination of serum metanephrines in the diagnosis of pheochromocytoma]. Ann Endocrinol (Paris) 1994 54 337-42. 

Orsulak PJ, Kazuka P, Grab E, Schddkraut JJ. Determination of urinary normetanephrine and metanephrine by radial-compression Uquid chromatography and electrochemical detection. Clin Chem 1983 29 305-9. 

Shoup RE, Kissinger PT. Determination of urinary normetanephrine, metanephrine, and 3-methoxytyramine by liquid chromatography, with amperometric detection. Clin Chem 1977 23 1268-74. 

Taniguchi K, Kakimoto Y, Armstrong M. Quantitative determination of metanephrine and normetanephrine in urine. J Lab Clin Med 1964 64 469-84. 

WHson SP, Kamin DL, Feldman JM. Acetaminophen administration interferes with urinary metanephrine (and catecholamine) determinations. Clin Chem 1985 31 1093-4. 

Chan et developed an assay for the simultaneous determination of catecholamines and metanephrines using FMOC derivatization. The assay is convenient for the simultaneous analysis of NM, MN, E and DA in human urine sample without prior extraction procedures. In this study, urine was directly derivatized and subjected to a simple extraction step with... 

S. Bninjes, D. Wyhenga and V.J. Johns, Jr, Fluorometric determination of urinary metanephrine and normetanephrine, Clin. Chem., 10, 1-12 (1964). 

D. Chou, M. Tsuru, J.L. Holtzman and J.H. Eckfeldt, Interference by the 4-hydroxylated metabolite of propranolol with determination of metanephrines by the Pisano method, Clin. Chem., 26, TN,-TT1 (1980). 

N.G. Abeling, A.H. van Gennip, H. Overmars and P.A. Voute, Simultaneous determination of catecholamines and metanephrines in urine by HPLC with fluorometric detection, Clin. Chim. Acta, 137, 211-226 (1984). 

J. Jouve, N. Mariotte, C. Sureau and H.P. Muh, High-performance liquid chromatography with electrochemical detection for the simultaneous determination of the methoxy lated amines, normetanephrine, metanephrine and 3-methoxytyramine in urine, J. Chromatogr, llA, 53-62 (1983). 

Determination of Urinary 3-Methoxy-tyramine, Normetanephrine, and Metanephrine in Pheoochrocytoma and Neuroblaistoma by Gas Chromatography... 

Using these procedures, it has been shown that about 9 per cent of the excreted VMA is formed from adrenaline and 91 per cent by a route that does not include adrenaline as an intermediate. Furthermore, 46 per cent of the VMA formed from adrenaline was formed via metanephrine [336]. Similar techniques have been applied in determining the rates of formation and excretion of all the various products of noradrenaline and adrenaline metabolism [337]. 

A sensitive spectrophotometric method for the determination of urinary noradrenaline, adrenaline, normetanephrine, metanephrine and dopamine involving the formation of aminochrome-sodium bisulphite addition products has been described [197] and is based on an earlier method for noradrenaline and adrenaline [198]. 

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