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Medical applications separation techniques

Also, specific chapters deal with the use of CL reactions as detection mode in FIA (Chapter 12), in separational techniques, such as liquid chromatography (LC) (Chapter 14) or capillary electrophoresis (CE) (Chapter 15), in immunoassay (Chapter 18), and in the development of sensors (Chapter 20). The recent use of this technique for the analysis of DNA (Chapter 19) and a photosensitized CL mode for medical routine and industrial applications (Chapter 17) are also considered in this book. [Pg.60]

Solution-cast film is produced on a larger scale for medical applications, battery separators, or other specialty uses with machinery of the type shown in Figure 3.2 [2], Viscous film is made by this technique. The solution is cast onto the surface of a rotating drum or a continuous polished stainless steel belt. These machines are generally enclosed to control water vapor pickup by the film as it dries and to minimize solvent vapor losses to the atmosphere. [Pg.91]

In summary we underline the fact that as far as urine analysis is concerned, and this is also true for blood, the most satisfactory results published so far are those obtained with Moore and Stein s 1954 procedure (see (b) Dowex 50-X4 columns) whereas the two latest improvements in technique open the way to much wider investigation in this field of biochemical and medical research. The technique is also applicable for the determination of a single amino acid, adequate shortcuts and simplifications in the procedure being introduced accordingly. As examples, we shall mention that appropriate rapid operations have been described for the separation on ion exchange columns and subsequent determination of (3-alanine (C20), (3-aminoisobutyric acid (E5) or y-aminobutyric acid (W1). Similar adjustments may also be made for other amino acids, such as the basic amino acids, for instance, with Moore and Stein s 1954 procedure (M15). [Pg.215]

Hollow membrane fibers are required for many medical application, e.g. for disposable dialysis. Such fibers are made by usmg an appropriate fiber spinning technique with a special inlet in the center of the spinneret through which the fiber core forming medium (liquid or gas) is injected. The membrane material may be made by melt-spinning, chemical activated spinning or phase separation. The thin wall (15-500 xm thickness) acts as a semi-permeable membrane. Commonly, such fibers are made of cellulose-based membrane materials such as cellulose nitrate, or polyacrylonitrile, polymethylmethacrylate, polyamide and polypropylene (van Stone, 1985). [Pg.100]

In 1861, Thomas Graham Bell in Glasgow, Scotland, carried out the first dialysis experiments (and coined the term dialysis ), separating crystalloids and cohoids in a solution. BeU predicted that this technique could have medical application, but this was not realized until nearly 100 years later in the work of Willem Kolff and then Belding Scribner, who made HD a feasible treatment in the early 1960s. Since then, HD and more recently PD have extended the lives of many people, sometimes for up to 20 or 30 years. [Pg.1719]

New technology opens up completely new possibilities to design 3D structures for medical applications. The hexagonal braiding technique developed by Schreiber et al. (2009) allows various shapes such as bifurcations or stent into the stent to be produced. Such braided multilayer structures create mechanically separated layers on the outside and an inner structure that facilitates, for example, a functional respiratory epithelium to be established and make it possible to maintain the mucociliary function of the stented area in treatment of lung cancer (PulmoStent, 2014). [Pg.325]

The majority of the HA producing technologies has focused mainly on obtaining a highly pure polymer suitable for clinical applications [19]. Several separation techniques such as protease digestion, HA ion-pair precipitation, membrane ultrafiltration, HA non-solvent precipitation and lyophilisation [20,21] have been used to obtain the pnre compound. However, an economically appropriate and simple method is still needed for the production of high grade and pure HA for medical applications. [Pg.106]

The development of hyphenated techniques for rapid separation of mixtures and spectroscopic analysis of its components is a topic of great interest, particularly when the amount of sample is limited and the need for automatization is high. This is the case for high-pressure liquid chromatography nuclear magnetic resonance-mass spectroscopy (HPLC NMR-MS), which has been shown to be extremely useful in certain pharmaceutical and medical applications. [Pg.93]

Morf and W. Simon, Liquid membrane ion-selective electrodes and their biomedical applications. Chapter 2 of Medical and Biological Applications of Electrochemical Devices (ed. J. Koryta), John Wiley Sons, Chichester (1980). MS - mixed solution technique, SS - separate solution technique symbols for ion-exchanging ions from table 7.1. [Pg.217]

In past years, on line chromatographic coupling techniques such as HPLC and CE coupled to ICP-MS with the isotope dilution technique have been used for element quantification in speciation analysis. An interesting application of the isotope dilution technique in medical research was proposed recently by Prange and co-workers, who added highly enriched " S, Cu, Zn and Cd spikes to the interface of the CE-ICP-MS system. The authors separated isoforms of metallothionein (e.g., of rabbit liver) by capillary electrophoresis and quantified S, Cd, Cu and Zn concentrations in isoforms by ICP-SFMS using the isotope dilution technique. A new selenized yeast reference material (SELM-1) for methionine, selenomethionine (SeMet) and total selenium content has also certified by an intercomparison exercise. ... [Pg.198]

The application of microanalytical techniques, such as flow injection in ICP-MS (FI-ICP-SFMS), is also of special interest in medical research where very small sample volumes have to be characterized, e.g. for Cr determination in DNA by sector-field ICP-MS.For the separation of isobaric interferences of Cr and Ar C the measurements were performed at a mass resolution of m/Am = 3000. Transient signals of Cr and Cr analyzed by FI-ICP-SFMS of a I0p,gl chromium solution (sample loop 20p,l) are illustrated in Figure 9.31a. For quantification by the isotope dilution technique, the small volume of DNA available (diluted 1 10) was injected into a continuous flow of 2 % HNO3, which is spiked with high-enriched Cr solution (the isotope abundance of Cr was 83.4%). The application of the isotope dilution technique in flow injection ICP-SFMS is shown in Fig. 9.31 b. ... [Pg.353]


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