Matrix-assisted laser desorption ionization data acquisition

Also, the modified peptides may ionize less efficiently by electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) than those of the regular, unmodified peptides in a complex mixture, and the low-abundance ions of modified peptides that coelute with intense ions of unmodified peptides may be missed by conventional data-dependent acquisition [2]. 

Ruh, H., Sandhoff, R., Meyer, B., Gretz, N., and Hopf, C. 2013. Quantitative characterization of tissue globotetraosylceramides in a rat model of polycystic kidney disease by primadrop sample preparation and indirect high-performance thin layer chromatography-matrix-assisted laser desorption/ionization-time-of flight-mass spectrometry with automated data acquisition. Anal. Chem., 85 6233-6240. 

Sehumaker S, Borror CM, Sandrin TR. Automating data acquisition aifects mass spectrum quality and reproducibility during bacterial profiling using an intact cell sample preparation method with matrix-assisted laser desorption/ionization time-of- light mass speetrometry. Rapid Com-mun Mass Sp. 2012 26(3) 243-53. 

Key words Mass spectrometry imaging, matrix-assisted laser desorption/ionization, nervous tissue, pituitary, mouse, stretched sample, image reconstruction, automated data acquisition. 

With the introduction of fast-atom bombardment (FAB) in 1982, and matrix-assisted laser desorption/ ionization (MALDI) and electrospray ionization (ESI), most of the biomedical applications have been directed towards these methods. The 52( f.pD method has been found to have wide applicability, including the study of refractory materials, catalysts, semiconductors and frozen gases. Electronics capable of measuring the timing of events with subnanosecond resolution (the time it takes for a single photon to travel 1 cm) is used by this method as well as event-by-event data acquisition using the computer to make decisions at the molecular level, the basis of correlation mass spectrometry, a unique feature of 252Cf-PD. 

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