Master plate

Fig. 1. Lacquer-coated optical readout laser disk master. Plating by (a) electroless silver spray coating and by (b) vacuum evaporation. Scale bar, cm.

A Petri dish containing bacterial colonies is blotted with nitrocellulose paper. This transfers a large portion of each colony to the paper, which is saturated with a solution that lyses (breaks open) the cells. The DNA of the lysed colonies is denatured with alkali. The nitrocellulose paper is neutralized, washed, and the paper either baked in an oven or treated with ultraviolet light to immobilize the denatured DNA. The DNA on the paper is hybridized with the labeled probe of interest, and the excess label is washed off. The dried paper is exposed to photographic film and the film developed. The exposed spots on the film can be matched with the colonies on the master plate and colonies picked off for further study. 

Synthesis and Properties of Photopolymer Printing Plates for a Printing Master Plate by Modification of Polyvinyl Alcohol... 

Therefore, metal plates have been conventionally used. We have performed the research and development of a photopolymer printing plate for a master plate with a new basic structure by combining an oligomer of urea structure having a polyvinyl base with polyvinyl alcohol, photopolymerization initiator and other ingredients. The result shows that the newly developed plate (6) is so good that it has replaced metal plates and has been stably used at leading newspaper companies in Japan where several millions of newspapers are daily issued. 

Manufacture of Photopolymer Plate used as Master Plate for Duplicate Plate... 

When the hardness has reached this level, the plate can be a practical printing master plate for making paper mold and matrix. 

The Photopolymer Plate of over 85° Shore D hardness can stand temperatures over 160°C, and it could be successfully used as a master plate for making thermoformed matrix of phenol group resin to be used for thermoformed polymer printing plates such as rubber plates and the like under conditions of 30 kg/cm2 pressure... 

The Photopolymer Plate, a water developable photopolymer relief printing master plate made by modification of polyvinyl alcohol with urea group oligomer having a functional polyvinyl base, for making paper mold and matrix for printing master plates, has characteristics which are very close to those of the conventionally etched metal relief printing plates and has some superior points to them. 

Master plate colonies growing on ampicillin-containing medium... 

This pattern can be compared with that of the master plate to identify icyair... 

Invert the filter and gently lay it onto fresh media just prior to lysis to create a replica of the colony pattern of the filter. (Mark the plate to indicate the orientation of the filter on it) After an appropriate incubation, this becomes a master plate from which viable analogues of desirable colonies, as identified on the filter after hybridization and processing, can be recovered... 

Replica plating. A technique in which an impression of a culture is taken from a master plate and transferred to a fresh plate. The impression can be of bacterial clones or phage plaques. 

Using the same toothpicks transfer single colonies onto both a LB/Tet/Kan agar master plate and to a NCM on LB/Tet/Kan agar. 

Put the master plates in a refrigerator (these can be stored for several weeks). 

FIGURE 1.14. Schematic representation of the pattern replication process. The topography of the top plate induced a lateral force gradient that focuses the instability towards the downward pointing protrusions of the master plate. 

Fig. 7.6. (a) Standard screen-printing equipment, (b) Master plates leaving the in-line drying furnace after screen-printing, (c) Master plates after sintering in a batch oven... 

Further process steps such as dye coloration, electrolyte filling and seal-ing/lamination, leading to sealed completed modules, have also been carried out on 10 x 10 cm2 substrates. For these process steps, dedicated equipment has been developed from the laboratory stage, since it is not commercially available. A photo of a fully processed master plate is shown in Fig. 7.7. It contains 4 modules of 5 cells each (total area of 1 module 20 cm2) on one TCO plate of 100 cm2. These modules were intended for LCD powered price tags on supermarket shelves. 

Fig. 7.7. Fully processed master plate (10 x 10 cm2), containing 4 mini-modules. The dye used is N719 [Ru(NCS)2(2,2 -bipyridyl-4,4 -dicarboxylate)2]- A molten salt, hexylmethylimidazolium iodide (HMII) containing 10 mM I2, was used as the electrolyte...

Aliquots of the selected clones should at this stage, be frozen down at -80°C, in complete medium containing 10% DMSO, and then transferred to liquid nitrogen. Any unpicked clones from the master plate may also be stored frozen. 

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