Mass spectrometry biomolecular interaction analysis

Nelson, R. W., Krone, J. R., and Jansson, O. (1997). Surface plasmon resonance biomolecular interaction analysis mass spectrometry. 1. Chip-based analysis. Anal. Biochem. 69, 4363-4368. 

C. Fenselau, MALDI MS and Strategies for Protein Analysis, Anal. Chem. 1997,69, 661 A R. W. Nelson, D. Nedelkov, and K. A. Tubbs, Biomolecular Interaction Analysis Mass Spectrometery, AnaL Chem. 2000, 72, 405A J. J. Thomas, R. Bakhtiar, and G. Siuzdak, Mass Spectrometry in Viral Proteomics, Acc. Chem. Res. 2000,33, 179 A. P. Snyder, Interpreting Protein Mass Spectra (Washington, DC American Chemical Society, 2000) S. C. Moyer and R. J. Cotter, Atmospheric Pressure MALDI, Anal. Chem. 2002, 74, 469A. 

Nelson RW, Krone JR. Advances in surface plasmon resonance biomolecular interaction analysis mass spectrometry (BIA/MS). J Mol Recognit 1999 12 77-93. 

Hashimoto S, Isobe T, Natsume T (2007) Biomolecular interaction analysis coupled with mass spectrometry to detect interacting proteins. In Walker JM (ed) The proteomics protocols handbook. Humana Press, Totowa, NJ... 

It is a feature of current developments in mass spectrometry that this method of analysis can be coupled or interfaced with other techniques. The interfacing may in fact be with further MS equipment (MS/MS), or with various separation techniques such as high-performance liquid chromatography (LC/MS), gas chromatography (GC/MS), electrophoresis, particularly high-resolution capillary electrophoresis (CE/ MS), and biomolecular interaction analysis (BIA/MS) (Krone 1997). 

Interfacing Biomolecular Interaction Analysis with Mass Spectrometry and... 

Over the past few years we have been developing new mass spectrometric approaches for the rapid, sensitive, and, accurate characterization of proteins. Reported here are some of our findings on the interfacing of Biomolecular Interaction Analysis with mass spectrometry, and the use of enzymatically active - or bioreactive - mass spectrometer probe tips in the characterization of analytes. 

Fig. 1 Biomolecular interaction analysis/mass spectrometry (BIA/MS). Biosensor chips are derivatized with affinant (or used with an affinant of streptavidin) and used in the BIA analysis of biological fluids. The chips are then introduced into a MALDI time-of-flight mass spectrometer and retained ligands analyzed by virtue of molecular weight.

Nelson, R.W. Nedelkov, D. Tubbs, K.A. Biomolecular Interaction Analysis Mass Spectrometry, Anal. Chem. 72,404A l 1A (2000). 

Nelson, R.W. Krone, J.R. Advances in Surface Plasmon Resonance Biomolecular Interaction Analysis Mass Spectrometry (BIA/MS), J. Mol. Recognit. 12, 77-93 (1999). 

Krone, J.R., Nelson, R.W., Dogruel, D., Withams, P., Granzow, R., BIA/MS interfacing biomolecular interaction analysis with mass spectrometry, AnoZ. Biochem., 244,124—132, 1997. 

Sonksen, C.P., Nordhoff, E., Jansson, O., Mahnqvist, M., Roepstorff, R, Combining MALDI mass spectrometry and biomolecular interaction analysis using a biomolecular interaction analysis instrument. Ana/. Chem., 70, 2731-2736,1998. 

Nedelkov D, Nelson RW, Kiernan UA et al (2003) Detection ofbound and free IGF-1 and IGF-2 in human plasma via biomolecular interaction analysis mass spectrometry. FEBS Lett 536 130-134... 

Biomolecular interaction analysis-mass spectrometry (BIA-MS), a combination of SPR with MS, was first explored by Krone et al. in 1996 where SPR was used to quantify interactions between proteins and MS was used to determine the structural features of die bound proteins. Review articles on SPR-MS describe recent progress in the field of SPR-MS, including future trends and possible applications. ... 

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