Biomolecular mass spectrometry

Biological samples are potentially excellent records of their environment and are being examined by inorganic and biomolecular mass spectrometry to an increasing extent. Biomaterials such as carbonate shells, fish otoliths and fish scales or tree rings that increase their mass regularly on an annual basis may record changes in their environment via the concentrations of metals or non-metals and the isotopic composition of elements.2... 

To analyze element distribution, isotope ratios and species in single cells, development of advanced inorganic mass spectrometric techniques in combination with biomolecular mass spectrometry is required for future applications. 

Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands 2Institute of Biochemistry, University of Giessen, Friedrichstrasse 24, 35392 Giessen, Germany... 

Then, within a short period, both MALDI (matrix-assisted laser desorption ionization) and electrospray ionization were introduced and found widespread use in biomolecular mass spectrometry. As noted earlier, Koichi Tanaka (MALDI) and John Fenn (electrospray ionization) were awarded the 2002 Nobel Prize in Chemistry for these innovations. Both MALDI and electrospray ionization are considered to be soft ionization techniques that induce little, if any, molecular fragmentation. 

Ying Liu School of Biology, Georgia Institute of Technology, Atlanta, GA, USA A.F. Maarten Altelaar Biomolecular Mass Spectrometry and Proteomics Group, Utrecht University, Utrecht, The Netherlands... 

Nelson, R. W., Krone, J. R., and Jansson, O. (1997). Surface plasmon resonance biomolecular interaction analysis mass spectrometry. 1. Chip-based analysis. Anal. Biochem. 69, 4363-4368. 

Altelaar A, Klinkert I, Jalink K, et al. Gold-enhanced biomolecular surface imaging of cells and tissue by SIMS and MALDI mass spectrometry. Anal. Chem. 2006 78 734-742. 

R.P. Evershed, Biomolecular analysis by organic mass spectrometry, in Modern Analytical Methods in Art and Archaeology, E. Ciliberto and G. Spoto (Eds), Wiley Interscience, New York, 2000. 

Kaltashov, I.A. Eyles, S.J. Studies of biomolecular conformations and conformational dynamics by mass spectrometry. Mass Spectrom. Rev. 2002, 21, 37-71. 

C. Fenselau, MALDI MS and Strategies for Protein Analysis, Anal. Chem. 1997,69, 661 A R. W. Nelson, D. Nedelkov, and K. A. Tubbs, Biomolecular Interaction Analysis Mass Spectrometery, AnaL Chem. 2000, 72, 405A J. J. Thomas, R. Bakhtiar, and G. Siuzdak, Mass Spectrometry in Viral Proteomics, Acc. Chem. Res. 2000,33, 179 A. P. Snyder, Interpreting Protein Mass Spectra (Washington, DC American Chemical Society, 2000) S. C. Moyer and R. J. Cotter, Atmospheric Pressure MALDI, Anal. Chem. 2002, 74, 469A. 

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