Interactions biomolecular interaction analysis

Nelson, R. W., Krone, J. R., and Jansson, O. (1997). Surface plasmon resonance biomolecular interaction analysis mass spectrometry. 1. Chip-based analysis. Anal. Biochem. 69, 4363-4368. 

So far, optical biosensors have been mostly used in research laboratories for biomolecular interaction analysis in which the binding interactions between a ligand immobilized at the sensor surface and its molecular partner in a solution passed over this surface are studied. 

Goddard, N. J. Pollard Knight, D. Maule, C., Real time biomolecular interaction analysis using the resonant mirror sensor, Analyst 1994, 119, 583 588... 

C. Fenselau, MALDI MS and Strategies for Protein Analysis, Anal. Chem. 1997,69, 661 A R. W. Nelson, D. Nedelkov, and K. A. Tubbs, Biomolecular Interaction Analysis Mass Spectrometery, AnaL Chem. 2000, 72, 405A J. J. Thomas, R. Bakhtiar, and G. Siuzdak, Mass Spectrometry in Viral Proteomics, Acc. Chem. Res. 2000,33, 179 A. P. Snyder, Interpreting Protein Mass Spectra (Washington, DC American Chemical Society, 2000) S. C. Moyer and R. J. Cotter, Atmospheric Pressure MALDI, Anal. Chem. 2002, 74, 469A. 

Sjoelander, S., Urbaniczky, C., Integrated fluid handling system for biomolecular interaction analysis. Anal. Chem. 1991, 63, 2338-2345. 

Nelson RW, Krone JR. Advances in surface plasmon resonance biomolecular interaction analysis mass spectrometry (BIA/MS). J Mol Recognit 1999 12 77-93. 

Malmqvist M. Epitope mapping by label-free biomolecular interaction analysis. Methods 1996 9 525-532. 

Hashimoto S, Isobe T, Natsume T (2007) Biomolecular interaction analysis coupled with mass spectrometry to detect interacting proteins. In Walker JM (ed) The proteomics protocols handbook. Humana Press, Totowa, NJ... 

Herberg, F. W., Zimmermann, B. (1999). Analysis of protein kinase interactions using biomolecular interaction analysis. In Hardie, D. G. (Ed.), Protein Phosphorylation - A Practical Approach. Oxford University Press, pp. 335-371. 

Zimmermann, B., Hahnefeld, C., Herberg, F.W. (2002). Applications of biomolecular interaction analysis in drug development. Targets 1, 66-73. 

It is a feature of current developments in mass spectrometry that this method of analysis can be coupled or interfaced with other techniques. The interfacing may in fact be with further MS equipment (MS/MS), or with various separation techniques such as high-performance liquid chromatography (LC/MS), gas chromatography (GC/MS), electrophoresis, particularly high-resolution capillary electrophoresis (CE/ MS), and biomolecular interaction analysis (BIA/MS) (Krone 1997). 

Biomolecular interaction analysis (BIA) is a relatively new technique which enables the detection of biomolecules and the monitoring of interactions between two or more species to be carried out in real time, without the use of labels. The detection principle used depends on the phenomenon of Surface Plasmon Resonance (SPR). 

Interfacing Biomolecular Interaction Analysis with Mass Spectrometry and... 

Over the past few years we have been developing new mass spectrometric approaches for the rapid, sensitive, and, accurate characterization of proteins. Reported here are some of our findings on the interfacing of Biomolecular Interaction Analysis with mass spectrometry, and the use of enzymatically active - or bioreactive - mass spectrometer probe tips in the characterization of analytes. 

Fig. 1 Biomolecular interaction analysis/mass spectrometry (BIA/MS). Biosensor chips are derivatized with affinant (or used with an affinant of streptavidin) and used in the BIA analysis of biological fluids. The chips are then introduced into a MALDI time-of-flight mass spectrometer and retained ligands analyzed by virtue of molecular weight.

Biomolecular Interaction Analysis with MS and MS Probe Tips 495... 

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