Sensitivity analysis mass spectrometric imaging

Besides sensitive methods for the analysis of proteins, bioinformatics is one of the key components of proteome research. This includes software to monitor and quantify the separation of complex samples, e.g., to analyze 2DE images. Web-based database search engines are available to compare experimentally measured peptide masses or sequence ions of protein digests with theoretical values of peptides derived from protein sequences. Websites for database searching with mass spectrometric data may be found at http //www.expasy.ch/tools, http //prospector.ucsf. edu/ and http //www.matrixscience.com. 

In 2006, Lobinski et al.1 reported on the imaging and speciation analysis of trace elements to study the element distribution, oxidation state, metal site and metal structure in biological environments using mass spectrometric techniques (LA-ICP-MS, SIMS, MALDI-MS) and non-mass-spectrometric techniques such as micro-PIXE (proton induced X-ray emission), XANES (X-ray absorption near edge structure) and EXAFS (extended X-ray absorption fine structure) -the latter two techniques are very sensitive due the use of a more intense synchrotron beam.1... 

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