Mannitol identification

Polarimetric analysis of sorbitol and mannitol in the presence of each other and of sugars is possible because of their enhanced optical rotation when molybdate complexes are formed and the higher rotation of the mannitol molybdate complex under conditions of low acidity (194). The concentration of a pure solution of sorbitol may be determined by means of the refractometer (195). Mass spectra of trimethylsilyl ethers of sugar alcohols provide unambiguous identification of tetritols, pentitols, and hexitols and permit determination of molecular weight (196). 

An alternative method for assessing cell layer integrity is through the use of hydrophilic paracellular transport markers (e.g., radiolabeled D-mannitol or fluorescein-Na+), which passively traverse cells by the paracellular route. Small amounts of compound required for in vitro conjunctival cell culture transport experiments make this approach well suited for screening purposes. Relative absorption index of a series of pharmacologically active molecules can be ranked against known markers for the identification of candidates with potential absorption problems, which is a reliable tool to select drug candidates with optimal characteristics. 

Reference alditol acetates elute from the BPX-70 column in the following order erythritol triacetate, 2-deoxyribitol tetraacetate, rhamnitolpentaacetate, fucitolpentaacetate, ribitol pentaacetate, arabinitol pentaacetate, xylitol pentaacetate, 2-deoxyglucitol hexaacetate, allitol hexaacetate, mannitol hexaacetate, galactitol hexaacetate, glucitol hexaacetate, and rnyo-inositol hexaacetate. Their identification can be verified by using individual standards. 

When 15 mM /Va-formyl-/Ve-fructosyl-lysine (fFL) in 0.2 M phosphate buffer, pH 7.4, was incubated in air for 15 d at 37 °C, not only was CML formed, but a substantial amount of lysine was recovered, which N. Ahmed el al.50 and M.U. Ahmed el al.51 interpreted as due to reversal of the Amadori rearrangement. The reduction of the reaction mixture with NaBH4 and subsequent identification of both glucitol and mannitol supports the interpretation. 

The inner etherification induced in the sorbitol and D-mannitol structures during polyester formation, made a study of their inner ethers or anhydrides a matter of practical significance. A new monoanhydride of sorbitol was described (21) and designated arlitan (from the initials of the Atlas Research Laboratory). Identification as 1,4-anhydro-D-glucitol was made by comparison of its sirupy tetramethyl ether with that obtained by acid anhydrization of Irvine s 2,3,5,6-tetramethyl-D-glu-citoP by oxidation of the tetramethyl ether to dimethyl-L-threaric acid [0-dimethyl-L-((iea tartaric acid] and identification as the crystalline diamide and by its behavior on oxidation with lead tetraacetate. ... 

The presence of mannitol at the proximal end of the oligosaccharides and the identification of the modified derivatives of serine and threonine in the peptide moieties... 

Davis G., Garwood V., Barfuss D.L., Husaini S.A., Blanc M.B. and Viani R. (1990) Chromatographic profile of carbohydrates in commercial coffees. 2. Identification of mannitol. J. Agric. Food Chem. 38, 1347-50. 

Malic enzyme, labeling of, 139 Mandelate racemase, 541-548 effect of metal ion on, 546, 547 residue identification, 547, 548 stoichiometry of binding, 545, 546 D-Mannitol, 384-386 Membrane, see abo specific type sodium channels, 288 transport, 167 Menadione, 76 Mercaptans, 317 2-Mercaptobenzothiazole, 540 Mercaptoethanol, 402, 451 6-Mercaptopurine, analogs of nucleotides, 289... 

Guddat, S., Thevis, M., and Schanzer, W. (2008) Identification and quantification of the osmodiuretic mannitol in urine for sports drug testing using gas chromatography-mass spectrometry. European Journal of Mass... 

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