Lyophilisate

The Na salt is prepared by dissolving the in H2O containing 2 mols of NaOH per mol of acid reagent and lyophilising. It complexes with Ca and Mg ions. 

Acetoin dehydrogenase [from beef liver acetoin NAD oxidoreductase] [9028-49-3] Mr 76000, [EC 1.1.1.5]. Purified via the acetone cake then Ca-phosphate gel filtration (unabsorbed), lyophilised and then fractionated through a DEAE-22 cellulose column. The Km for diacetyl in 40pM and for... 

Corticotropin [92307-52-3] polypeptide Mr -4697. Extract separated by ion-exchange on CM-cellulose, desalted, evapd and lyophilised. Then run on gel filtration (Sephadex G-50) [Lande et al. Biochemical Preparations 13 45 1971 Esch et al. Biochem Biophys Res Commun 122 899 1984],... 

Deoxyribonucleic acid (from plasmids). Purified by two buoyant density ultracentrifugations using ethidium bromide-CsCl. The ethidium bromide was extracted with Et20 and the DNA was dialysed against buffered EDTA and lyophilised. [Marmur and Doty J Mol Biol 5 109 1962 Guerry et al. J Bacteriol II6 1064 1973.] See p. 504. 

Interleukin (from human source). Purified using lyophilisation and desalting on a Bio-Rad P-6DC desalting gel, then two steps of HPLC, first with hydroxylapatite, followed by a TSK-125 size exclusion column. [Kock and Luger J Chromatogr 296 293 7984 ]... 

Microbial strains must be maintained in such a way that they do not lose their desirable characteristics. Some strains are maintained by regular subculturing, whereas others are lyophilised (freeze-dried), or frozen under nitrogen, or held at -80°C in a freezer. To ensure that a standard inoculum can be obtained on demand, great care is taken to ensure that foe stored cultures are pure and foe viability is known. 

Initially fermentation broth has to be characterised on the viscosity of the fluid. If the presence of the biomass or cells causes trouble, they have to be removed. Tire product is stored inside the cells, the cells must be ruptured and the product must be freed. Intracellular protein can easily be precipitated, settled or filtered. In fact the product in diluted broth may not be economical enough for efficient recovery. Enrichment of the product from the bioreactor effluents for increasing product concentration may reduce the cost of product recovery. There are several economical methods for pure product recovery, such as crystallisation of the product from the concentrated broth or liquid phase. Even small amounts of cellular proteins can be lyophilised or dried from crude solution of biological products such as hormone or enzymes.2,3... 

Liquid-liquid extraction, 172, 183 Logistic model, 55, 56 Lyophilisation, 172 Lysine, 1, 8, 202, 339, 340... 

The course of separation of the lyophilisate from pulp by this method was very similar to separation of the juice lyophilisate but led to only one activity peak. The pH optima determination (Fig. 2) and gel chromatography on Superose 12 (Fig. 3 a,b,c) showed these fractions to be partially purified, complex mixture of more exopolygalacturonase forms with different molecular masses and pH optima. 

Pendlington aw, Meuree-Vanlaethem N, Brookes A (i<)g6) The Method Specific Certification of the Mass Fraction of Dietary Fibre in Lyophilised Haricot Beans, Carrot, Apple, Full Fat Soya Flour and Bran Breakfast Cereal Reference Materials CRMs 514, 515, 516, 317 and 518. European Commission Report EUR 17451 EN, Luxembourg. 

Voilley, A., Sauvageot, F., Simatos, D. Confidents de volatilit, r,lative et retention au cours de la lyophilisation de quelques alcools, p. 639-647. International Institute of Refrigeration (Washington, 1971)... 

Brinkhoff, O. Primarpackmittel fur Lyophilisate, p. 145 Essig-Oschmann, Lyophilisation, Bd. 35. Copyright Wissenschaftliche Verlagsgesellschaft., Stuttgart 1993... 

Willemer, H. Moderne Anlagen der Lyophilisation in Essig-Oschmann Lyophilisation, Wis-senschaftliche Verlagsgesellschaft mbH, Stuttgart, vol. 35, 1993... 

Willemer, H. Influence of product temperature and gas composition on the lyophilisation process. International Congress, p. 63-77, Basel 1994, Copyright 1994 PDA, Inc., Bethesda, Maryland, USA... 

Steiner, R. VHP -Sterilisation of freeze-dryers. ISPE-Seminar Lyophilisation, Antwerp, Nov. 1994. Internnational Society of Pharmaceutical Engineering (ISPE). 

Hyatt, G. W. Precedes employes pour obtenir des tissus humain a usage chirurgical et, en particulier methode de conversation par lyophilisation, p. 279-301... 

Bassett, C. A. L. A survey of the current status of tissue procurement, processing and use. Aspect theoriques et industriels de la lyophilisation, p. 332-339, Hermann, Paris VI, 1964... 

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