Lycopene accumulation

FIGURE 5.3.5 Enhancement of lycopene accumulation in . coZi by over-expression of DXS. Lycopene accumulation (left) is enhanced (right) when E. coli cells carrying a carotenoid pathway gene cassette (+EIB) are further transformed with a dxs gene on a multicopy plasmid (+E1B +dxs). Lycopene hyperaccumulation was demonstrated by Matthews and Wurtzel. ... 

Bowen, P, L. Chen, M. Stacewicz-Sapuntzakis et al. 2002. Tomato sauce supplementation and prostate cancer Lycopene accumulation and modulation of biomarkers of carcinogenesis. Exp Biol Med (Maywood) 227(10) 886-893. 

In 1969, Knypl (10) found that an onium compound, chlorme-quat [(2-chloroethyl)-trimethylammonium chloride], caused all-trans-lycopene accumulation in detached pumpkin (Curcubita pepo) cotyledons. However, no such response was observed when chlorme-quat was applied to other plant tissues (11). A major step forward was observed in 1970 with the discovery that another onium compound, 2-(4-chlorophenylthio)-triethylammonium chloride (CPTA), can cause the accumulation of lycopene in a wide array of plant tissues and microorganisms (11). 

Amitrole (3-amino-lH-l,2,4-triazole) has been known to lead to some lycopene accumulation in vivo at a temperature-dependent rate but it is not considered to... 

Lycopene accumulation induced by 2-(4-chloro-phenyl-thio)-triethylamine hydrochloride,... 

Beta modifier mo modifies the expression of Beta. In BB mog mos genotypes 90 % of the carotenoid content is present as p-carotene and the fruits are orange. On the other hand, in BB mob mos 50 % of the carotenoids are present as p-carotene and lycopene accumulates up to less than 50 % of total carotenoids, resulting in red-orange fmits [35]. 

It has been reported that the lycopene accumulation depends on temperature and seems to take place at a range of average day temperature between 12 C and 32 °C [125] or 35 °C [126], with the optimal conditions around 22-26 °C [127]. For p-carotene accumulation, the range of average day temperature is wider than for lycopene. Its biosynthesis is poorly affected by temperatures lower than 12 °C [128] and with temperatures higher than 35 °C when the lycopene accumulation is... 

Alba R, Cordonnier-Pratt MM, Pratt LH (2000) Fruit-localized phytochromes regulate lycopene accumulation independently of ethylene production in tomato. Plant Physiol... 

Kim, J.H. et al. (2005) Identification of genes affecting lycopene accumulation in Escherichia coli using a shot-gun method. Biotechnol. Bioeng.,... 

The fruit of the mutant hpl contains enhanced levels of carotenoids and sucrose in comparison to wild-type. Alba et aL demonstrated that fruit-locahzed phytochromes control lycopene accumulation in tomato pericarp during fruit ripening but do not regulate ethylene biosynthesis and many other aspects of fruit ripening in tomato. Their obvious conclusion was that fruit-localized phytochromes are not global regulators of tomato fruit ripening. 

Alba, R., Cordonnier-Pratt, M.-M., and Pratt, L.H., Fruit-localized phytochromes regulates lycopene accumulation independently of ethylene production in tomato. Plant Physiol, 123,363,2000. Trewavas, A.J. and Malhb, R., Signal perception and transduction the origin of the phenotype. Plant Cell 9,1181, 1997. 

---