Luminescence living cells

Fig. 2.3 Luminescence spectra of the living cells of luminous bacteria. F, P. fischeri P, P. phosphoreum (Eley etal., 1970) Y, P. fischeri, strain Y-l (Ruby and Nealson, 1977). Reproduced with permission from the American Chemical Society and AAAS.

Greer, L. F., Ill and Szalay, A. A. (2002). Imaging of light emission from the expression of luciferases in living cells and organisms A review. Luminescence 17, 43-74. 

Other applications dealt with the development of a luciferin ester substrate to measure the luciferase activity in living cells [141], the detection of toxic compounds such as sodium azide, fluoroacetic acid, and antibiotics [142], the development of a biosensor for the determination of bioavailable mercury [143], the use of eukaryotic luciferases as bacterial markers with different colors of luminescence [144], the determination of complement-mediated killing of bacteria [145], and the development of a bioassay for the determination of HIV type 1 virus and HIV-1 Tat protein activity, valuable also for analysis of HlV-inhibi-tory agents [146],... 

The long-lived phosphorescence of the tryptophan in alkaline phosphatase is unusual. Horie and Vanderkooi examined whether its phosphorescence could be detected in E. coli strains which are rich in alkaline phosphatase.(89) They observed phosphorescence at 20°C with a lifetime of 1.3 s, which is comparable to the lifetime of purified alkaline phosphatase (1.4 s). Long-lived luminescence was not observed from strains deficient in alkaline phosphatase. The temperature dependence of tryptophan phosphorescence in the living cells was slightly different from that for the purified enzyme, indicating an environmental effect. 

Chauvin, A.S., Comby, S., Song, B., et al. (2007) A polyoxyethylene-substituted bimetallic europium heUcate for luminescent staining of living cells. Chemistry -A European Journal, 13, 9515-9526. 

Bartlett DW, Davis ME (2006) Insights into the kinetics of siRNA-mediated gene silencing from live-cell and live-animal bio-luminescent imaging. Nucleic Acids Res 34 322-333... 

Suzuki H, Dosaka S, Ohashi-Hatta Y, Sugiyama T. Luminescence microscope for reporter assay of single live cells. In Szalay AA, Hill PJ, Kricka LJ, Stanley PE. eds. Bioluminescence and Chemiluminescence. Singapore World Scientific 2006 53-6. 

VISUALIZATION OF SEQUENTIAL RESPONSE IN INTRA CELLULAR SIGNAL TRANSDUCTION CASCADE BY FLUORESCENCE AND LUMINESCENCE IMAGING IN THE SAME LIVING CELL... 

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